FlyBase Allele Report: Dmel\Caf1-180[3]

General Information

Symbol

Dmel\Caf1-180³

Species

D. melanogaster

Name

FlyBase ID

FBal0279529

Feature type

allele

Associated gene

Dmel\Caf1-180

Associated Insertion(s)

P{?GT1}Caf1-180³

Carried in Construct

Also Known As

p180³

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Klapholz et al., 2009)

Progenitor genotype

P{GT1}BG02681

(Klapholz et al., 2009)

Associated Insertion(s)

P{?GT1}Caf1-180³

Cytology

Description

Imprecise excision of the progenitor insertion resulting in a deletion that removes sequence encoding the first 992 amino acid residues of Caf1-180. A portion of the original insertion remains.

(Klapholz et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle | larval stage

(Klapholz et al., 2009)

decreased occurrence of cell division | larval stage

(Klapholz et al., 2009)

lethal - all die before end of second instar larval stage

(Klapholz et al., 2009)

some die during second instar larval stage

(Klapholz et al., 2009)

Phenotype Manifest In

embryonic/larval salivary gland

(Klapholz et al., 2009)

imaginal disc

(Klapholz et al., 2009)

Detailed Description

Statement

Reference

Hemizygous males undergo normal embryonic development under rich food conditions, but die 48 hours after hatching as second instar larvae, when they are significantly smaller than their wild-type siblings. Imaginal discs are not detected in the mutant larvae. Mutant salivary glands contain a normal number of nuclei at 40 hours after hatching, however, the nuclei are smaller than normal. At 44 hours after hatching, incorporation of BrdU is reduced in mutant salivary glands compared to wild type.

Mutant larvae survive for at least 120 hours after hatching on amino acid-deprived food medium.

(Klapholz et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for asf1¹ has no effect on the ability of Caf1-180^{Scer\UAS.P\T.cKa} expressed under the control of Scer\GAL4^Act5C.PI to rescue the lethality of Caf1-180³/Y males.

Heterozygosity for asf1² reduces the ability of Caf1-180^{Scer\UAS.P\T.cKa} expressed under the control of Scer\GAL4^Act5C.PI to rescue the lethality of Caf1-180³/Y males.

(Klapholz et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Caf1-180³ is rescued by Scer\GAL4^Act5C.PI/Caf1-180^{UASp.Tag:FLAG}

(Klapholz et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Caf1-180³

p180³

(Horard et al., 2018, Roelens et al., 2017, Klapholz et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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