FlyBase Allele Report: Dmel\Grasp65[100.2]

General Information

Symbol

Dmel\Grasp65^100.2

Species

D. melanogaster

Name

FlyBase ID

FBal0280240

Feature type

allele

Associated gene

Dmel\Grasp65

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Schotman et al., 2008)

Mutagen

Delta2-3 transposase

(Schotman et al., 2008)

Progenitor genotype

P{EPgy2}EY00794

(Schotman et al., 2008)

Cytology

Description

Imprecise excision of P{EPgy2}EY00794 has resulted in an approximately 375bp deletion comprising the first exon and part of the first intron of Grasp65.

(Schotman et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing

(Schotman et al., 2008)

wing disc | pupal stage

(Schotman et al., 2008)

Detailed Description

Statement

Reference

Approximately 10% of homozygous Grasp65^100.2 mutant flies survive to adulthood. Of this 10%, 35% exhibit severe wing problems, sometimes displaying severe adhesion problems exemplified in collapsed wings and large blisters. The majority of pupal wing imaginal discs are also severely compromised.

The stage 10-11 follicular epithelium is disrupted in Grasp65^100.2 mutant ovaries, with defects including misshaped cells and occasional loss of cell-cell contact, leading to severe disruption but without loss of polarity.

(Schotman et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Grasp65^100.2 is rescued by Scer\GAL4^Act5C.PI/Grasp65^UAS.EGFP

(Schotman et al., 2008)

Comments

Expression of Grasp65^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Act5C.PI rescues the follicular epithelium defects seen in Grasp65^100.2 mutant ovaries. The wing disc phenotypes are also completely rescued.

(Schotman et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Grasp65^100.2

dgrasp^100.2

(Schotman et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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