FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\His2BUASp.GFP
Open Close
General Information
Symbol
Dmel\His2BUASp.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0280299
Feature type
allele
Associated gene
His2B
Associated Insertion(s)
Carried in Construct
P{UASp(FRT.His2B-GFP)His2B-mKO}
Key Links
Transgenic product class
wild_type
(Tran et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Tran et al., 2012)
Mutagen
in vitro construct
(Tran et al., 2012)
Progenitor genotype
Carried in construct
P{UASp(FRT.His2B-GFP)His2B-mKO}
Cytology
Description

UASp regulatory sequences are fused upstream of a FRT cassette containing the His2B coding region tagged with GFP. The cassette is followed by the His2B coding region tagged with the mKO fluorescent protein.

(Tran et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
His2B
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      In the absence of Scer\FRT, the T:Avic\GFP-tagged His2B coding region in P{UASp(FRT.His2B-GFP)His2B-mKO} is expressed under the control of the UASp sequences and there is little expression of the T:Zzzz\KOmKO-tagged His2B coding region which is downstream of the Scer\FRT cassette. In the presence of Scer\FRT, the Scer\FRT cassette containing the T:Avic\GFP-tagged His2B coding region is excised, allowing expression of the T:Zzzz\KOmKO-tagged His2B coding region under the control of the UASp sequences. This dual-colour method can be used to differentially label "old" versus "new" histones in cells.

      (Tran et al., 2012)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      His2BScer\UAS.P\T.T:Avic\GFP
      His2BUASp.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)