FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\orb2RBDΔAGFP
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General Information
Symbol
Dmel\orb2RBDΔAGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0280512
Feature type
allele
Associated gene
Dmel\orb2
Associated Insertion(s)
TI{TI}orb2RBDΔAGFP
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Krüttner et al., 2012)
Progenitor genotype
Associated Insertion(s)
TI{TI}orb2RBDΔAGFP
Cytology
Description

Amino acid replacement: Y492A.

(Krüttner et al., 2012)

Amino acid replacement: F494A.

(Krüttner et al., 2012)

A modified orb2 genomic fragment has been inserted into the attP site present in orb2attP via phiC31-mediated recombination. The resulting allele contains an insertion of a single nucleotide in the A isoform specific exon, which disrupts the the orb2A isoform but leaves the orb2B isoform (which is tagged at the C-terminal end with GFP) intact. In addition, four point mutations have been introduced into the orb2 RBD domain, resulting in the amino acid changes Y492A, F494A, R601A and F604A. A single mFRT11 site is present downstream of the orb2 coding sequence, and single attL and attR sites (generated during the recombination process) are also present.

(Krüttner et al., 2012)

Amino acid replacement: R601A.

(Krüttner et al., 2012)

Amino acid replacement: R604A.

(Krüttner et al., 2012)
Allele components
Component
Use(s)
Tagged with
GFP
Also carries
mFRT11
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      orb2RBDΔA.T:Avic\GFP/orb2::Mmus\Cpeb2RBD.T:Avic\GFP male flies have almost no long term 24 hour memory when exposed to a courtship conditioning paradigm. Unlike wild type flies, males that experience futile courtship of mated and therefore unreceptive females fail to suppress their courtship towards other mated females.

      (Krüttner et al., 2012)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      orb2RBD<up>*ΔA</up>
      (Krüttner et al., 2012)
      orb2RBDΔA.T:Avic\GFP
      orb2RBDΔAGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)