UASt regulatory sequences drive expression of the Sema1a extracellular and transmembrane domains followed by the fragment crystallizable region of human immunoglobulin G. The chimeric protein is tagged at the C-terminal end with Tag:MYC.

Sema1a^{mEC.Fc.UAS.Tag:MYC}/Scer\GAL4^sca-537.4 partially rescues Sema1a^k13702

Expression of Sema-1a^{mEC.Scer\UAS.T:Hsap\Fc-IgG,T:Hsap\MYC} under the control of Scer\GAL4^sca-537.4 strongly rescues the central nervous system guidance defects seen in Sema-1a^k13702 embryos.

Co-expression of Sema-1a^{mEC.Scer\UAS.T:Hsap\Fc-IgG,T:Hsap\MYC} and Sema-1a^{mICD.Scer\UAS} under the control of Scer\GAL4^sca-537.4 does not result in additional rescue of the ISNb defects seen in Sema-1a^k13702 embryos compared to either line expressed alone.

Co-expression of Sema-1a^{mEC.Scer\UAS.T:Hsap\Fc-IgG,T:Hsap\MYC} and Sema-1a^{mICD.Scer\UAS.T:Hsap\Fc-IgG} under the control of Scer\GAL4^sca-537.4 does not result in additional rescue of the ISNb defects seen in Sema-1a^k13702 embryos compared to either line expressed alone.