FlyBase Allele Report: Dmel\psidin[IG978]

General Information

Symbol

Dmel\psidin^IG978

Species

D. melanogaster

Name

FlyBase ID

FBal0280783

Feature type

allele

Associated gene

Dmel\psidin

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Stephan et al., 2012)

Progenitor genotype

Cytology

Description

Amino acid replacement: E320K.

(Stephan et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:20,024,093..20,024,093 [+]

Nucleotide change:

G20024093A

Amino acid change:

E320K | psidin-PA

Reported amino acid change:

E320K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Stephan et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | somatic clone

(Stephan et al., 2012)

Phenotype Manifest In

adult olfactory receptor neuron Or42a | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or47a | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or59c | somatic clone

(Stephan et al., 2012)

lamellipodium

(Stephan et al., 2012)

Detailed Description

Statement

Reference

Homozygous olfactory receptor neuron (ORN) clones result in defects in glomerulus targeting. Mutant Or47a-expressing ORNs frequently innervate a ventromedial glomerulus (in wild-type animals they target the dorsomedial DM3 glomerulus). Mutant Or42a and Or59c ORNs show a strong mistargeting phenotype. Mutant Or92a ORNs show a mistargeting phenotype.

Wild-type Or59c, Or42a and Or47a ORNs project normally in a psidin^IG978 background.

Neurons in primary neuron cultures derived from mutant embryos have significantly smaller lamellipodia and significantly longer neurites compared to wild-type controls.

(Stephan et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

psidin^IG978 has abnormal neuroanatomy | adult stage | somatic clone phenotype, non-enhanceable | somatic clone by Scer\GAL4^Act.PU/Naa20A^KK102646

(Stephan et al., 2012)

NOT suppressed by

Statement

Reference

psidin^IG978 has abnormal neuroanatomy | somatic clone | adult stage phenotype, non-suppressible | somatic clone by Scer\GAL4^Act.PU/BacA\p35^UAS.cUa

(Stephan et al., 2012)

Other

Statement

Reference

Naa20A^KK102646, Scer\GAL4^Act.PU, psidin^IG978 has abnormal neuroanatomy | somatic clone | adult stage phenotype

(Stephan et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

psidin^IG978 has adult olfactory receptor neuron Or42a | somatic clone phenotype, non-enhanceable | somatic clone by Scer\GAL4^Act.PU/Naa20A^KK102646

(Stephan et al., 2012)

psidin^IG978 has adult olfactory receptor neuron Or59c | somatic clone phenotype, non-enhanceable | somatic clone by Scer\GAL4^Act.PU/Naa20A^KK102646

(Stephan et al., 2012)

NOT suppressed by

Statement

Reference

psidin^IG978 has adult olfactory receptor neuron Or59c | somatic clone phenotype, non-suppressible | somatic clone by Scer\GAL4^Act.PU/BacA\p35^UAS.cUa

(Stephan et al., 2012)

Other

Statement

Reference

Naa20A^KK102646, Scer\GAL4^Act.PU, psidin^IG978 has adult olfactory receptor neuron Or42a | somatic clone phenotype

(Stephan et al., 2012)

Naa20A^KK102646, Scer\GAL4^Act.PU, psidin^IG978 has adult olfactory receptor neuron Or59c | somatic clone phenotype

(Stephan et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of NAA20^KK102646 under the control of Scer\GAL4^Act.PU in psidin^IG978 olfactory receptor neuron (ORN) clones results in a significant decrease in cell number of Or42a and Or59c-expressing ORNs. The axon mistargeting seen in Or42a and Or59c ORNs homozygous for psidin^IG978 is not enhanced by expression of NAA20^KK102646 under the control of Scer\GAL4^Act.PU.

(Stephan et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cUa under the control of Scer\GAL4^Act.PU does not rescue the axon mistargeting phenotypes seen in psidin^IG978 Or59c olfactory receptor neuron clones.

(Stephan et al., 2012)

Complementation and Rescue Data

Rescued by

psidin^IG978 is rescued by Scer\GAL4^Act.PU/psidin^UAS.Tag:HA

(Stephan et al., 2012)

Comments

Expression of psidin^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU rescues the axon mistargeting phenotypes seen in psidin^IG978 Or59c olfactory receptor neuron clones.

(Stephan et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

psidin^IG978

(Stephan et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close