A 2.4kb genomic fragment encompassing the pck transcribed region, as well as the 700bp upstream and 130bp downstream sequences. Tag:CS(TEVp) has been inserted between amino acids 217 and 218 of pck.
Co-expression of Zzzz\NIaScer\UAS.cHa under the control of Scer\GAL4btl.PS produces tracheal defects in flies expressing pckT:Zzzz\TEV.CS in a pckG0012 mutant background, phenocopying the pckG0012 mutant phenotype.
pckTag:CS(TEVp) rescues pckG0012
Expression of pckT:Zzzz\TEV.CS rescues the tracheal phenotypes seen in pckG0012 mutant embryos.