FlyBase Allele Report: Dmel\Bin1[R7-18]

General Information

Symbol

Dmel\Bin1^R7-18

Species

D. melanogaster

Name

FlyBase ID

FBal0281002

Feature type

allele

Associated gene

Dmel\Bin1

Associated Insertion(s)

P{?EP}Bin1^R7-18

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

heat sensitive loss of function allele

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Canudas et al., 2005)

heat sensitive loss of function allele

(Singh et al., 2005)

Mutagen

Delta2-3 transposase

(Singh et al., 2005)

Progenitor genotype

Bin1^EP3462

(Singh et al., 2005, Singh, 2002.3.20)

Associated Insertion(s)

P{?EP}Bin1^R7-18

Caused by aberration

Df(3R)Bin1^R7-18

Cytology

Description

Consists of a deletion that removes the 5' end of the Bin1 gene. Retains part of the original P{EP}sra^EP3462 inserted transposon.

(Singh et al., 2005)

Imprecise excision of P{EP}sra^EP3462 has removed the N-terminal 2/3 of Bin1 and 23bp of the 5' UTR of next ORF, sra.

(Singh and Hanes, 2004)

601 bp deletion from P{EP} insertion site. So to be exact it removes 448bp out of 633bp of the Bin1 gene.

(Singh, 2002.3.20)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:16,186,437..16,187,037 [-]

Comment:

Approximate boundaries of the Bin1^R7-18 deletion, resulting from excision of P{EP}sra^EP3462 and reported as removing 601 bases from the P insertion site including 448 of the 633 bases of Bin1.

(Singh and Hanes, 2004, Singh, 2002.3.20)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

enhancer of variegation, with P{UAS-lacZ.Abd-B.24F6}GCD-6/+:

(Canudas et al., 2005)

female fertile

(Singh et al., 2002)

lethal | heat sensitive

(Horner, 2006, Singh et al., 2005)

lethal | larval stage | recessive | heat sensitive

(Singh et al., 2005)

lethal | maternal effect

(Singh et al., 2005)

lethal | pupal stage | recessive | heat sensitive

(Singh et al., 2005)

male sterile

(Singh et al., 2002)

melanotic mass phenotype

(Singh et al., 2002)

viable

(Singh et al., 2005)

viable | poor

(Singh et al., 2002)

visible (with Df(3R)sbd45)

(Canudas et al., 2005)

visible | dominant

(Singh et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Approximately 80% of Bin1^R7-18/Df(3R)sbd45 males show transformation of A6 to A5, as judged by the presence of at least one bristle on the sixth sternite.

Bin1^R7-18/+ enhances the silencing of the w marker gene which is seen in the P{UAS-lacZ.Abd-B.24F6}GCD-6 insertion, such that the eye is less pigmented in the presence of Bin1^R7-18/+.

(Canudas et al., 2005)

Homozygous Bin1^R7-18 mutants are lethal, with arrest occurring most often during pupal stages of development. About 10% are also larval lethal. There is no embryonic lethality. The larval and pupal lethality is temperature sensitive; at 22^oC homozygotes hatch normally and develop into viable adults. Homozygous Bin1^R7-18 females produce eggs in which about 90% fail to develop. Although oogenesis appears normal, the eggs arrest prior to pronuclear fusion. At 29^oC progeny from heterozygous mothers display defects. Eggs develop but about 8% are embryonic lethal. the embryos have normal-looking cuticles and mouth hooks, but they die at late stages just prior to hatching as first instar larvae. Developing embryos from homozygous mutant germ-line clones show about 12% embryonic lethality at 25^oC which increases to 21% at 29^oC. These embryos typically have holes or patches of naked cuticle in the anterior and thoracic regions. In some embryos, the head skeleton is collapsed, and a smaller number of embryos have segmentation defects. Larvae that hatch survive to adulthood if they have wild-type Bin1 contributed from the male.

(Singh et al., 2005)

The frequency of homozygous mutants recovered is very low (~1 in 300). About half of the heterozygous adults show mutant wing phenotypes. A large percent of eggs from homozygous mothers burst upon removal of chorion. Almost all unhatched embryos have head defects. A small number of first instar larvae die after hatching and ~10% show segmentation defects. About half of the third instar larvae develop black masses and die as they pupate. Surviving adults show mild segmentation defects.

(Singh et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Bin1^R7-18 has lethal | heat sensitive phenotype, non-suppressible by sra^cHa

(Horner, 2006)

Suppressor of

Statement

Reference

Df(3R)sbd45/Bin1^R7-18 is a suppressor of visible | recessive phenotype of sd^Fab-X

(Canudas et al., 2005)

Bin1[+]/Bin1^R7-18 is a suppressor of visible | recessive phenotype of sd^Fab-X

(Canudas et al., 2005)

Other

Statement

Reference

Bin1^R7-18, sra[+]/sra^A108 has female semi-fertile phenotype

(Horner, 2006)

Bin1^R7-18, sra[+]/sra^A426 has female semi-fertile phenotype

(Horner, 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Bin1^R7-18, bcd⁶ has embryonic head | maternal effect phenotype, enhanceable by HDAC1⁰⁴⁵⁵⁶

(Singh et al., 2005)

Suppressor of

Statement

Reference

Df(3R)sbd45/Bin1^R7-18 is a suppressor of wing blade phenotype of sd^Fab-X

(Canudas et al., 2005)

Bin1[+]/Bin1^R7-18 is a suppressor of wing blade phenotype of sd^Fab-X

(Canudas et al., 2005)

Other

Statement

Reference

Bin1^R7-18, bcd⁶ has embryonic head | maternal effect phenotype

(Singh et al., 2005)

Bin1^R7-18, bcd⁶ has dorsal arm of apodeme of rostrum-haustellum joint | maternal effect phenotype

(Singh et al., 2005)

Bin1^R7-18, bcd⁶ has epipharyngeal sclerite | maternal effect phenotype

(Singh et al., 2005)

Bin1^R7-18, bcd⁶ has embryonic/larval mouth | maternal effect phenotype

(Singh et al., 2005)

Bin1^R7-18, bcd⁶ has dorsal bridge | maternal effect phenotype

(Singh et al., 2005)

Bin1^R7-18, bcd⁶ has median tooth | maternal effect phenotype

(Singh et al., 2005)

Bin1^R7-18, bcd⁶ has ventroposterior arm of apodeme of rostrum-haustellum joint | maternal effect phenotype

(Singh et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Bin1^R7-18/+, sra⁶⁸⁷/+ females are fertile, while Bin1^R7-18/+, sra^A108/+ and Bin1^R7-18/+, sra^A426/+ females show reduced fertility compared to control siblings.

The sra^cHa transgene does not rescue the Bin1^R7-18 mutation.

(Horner, 2006)

Trl^R67/Bin1^R7-18 and Rpd3^def24/Bin1^R7-18 transheterozygous males do not show transformation of A6 to A5.

The wing blade destruction phenotype of homozygous sd^Fab-X females is strongly suppressed by Bin1^R7-18/Df(3R)sbd45 : 13% of the double mutants show wing blade destruction in one wing, and 4% show wing blade destruction in both wings.

The wing blade destruction phenotype of homozygous sd^Fab-X females is significantly suppressed by Bin1^R7-18/+ (39% show wing blade destruction in one wing, 36% show wing blade destruction in both wings).

(Canudas et al., 2005)

9% of bcd⁶/Bin1^R7-18 double heterozygotes die as unhatched embryos with serious head defects including missing mouth parts. These larvae are missing labral structures most frequently the labrum itself and the epistomal sclerite. In some cases the dorsal bridge, dorsal bridge, dorsal and ventral arms, and post pharyngeal wall are absent or reduced in size.

(Singh et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Bin1[+]/Bin1^R7-18 is rescued by Bin1^+t4

(Singh et al., 2005)

Partially rescued by

Df(3R)sbd45/Bin1^R7-18 is partially rescued by Bin1^Tag:HA

(Canudas et al., 2005)

Comments

Bin1^T:Ivir\HA1 partially rescues the A6 to A5 transformation seen in Bin1^R7-18/Df(3R)sbd45 males, reducing the fraction of males that contain at least one bristle on the sixth sternite from approximately 80% to 50%, and also reducing the severity of the transformation (the frequency of males having more than 2 bristles on the sixth sternite is reduced from 34% to 4%).

(Canudas et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Bin1¹¹⁷

Bin1^R7-18

dsap18¹¹⁷

(Canudas et al., 2005)

dsap18^R7-18

(Canudas et al., 2005)

sap18^R7-18

(Horner, 2006)

Name Synonyms

Secondary FlyBase IDs

FBal0176374
FBal0134406

References (6)

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