FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ppkdeg.UAS.Tag:FLAG
Open Close
General Information
Symbol
Dmel\ppkdeg.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0281083
Feature type
allele
Associated gene
Dmel\ppk
Associated Insertion(s)
Carried in Construct
P{UAS-ppk.deg.FLAG}
Key Links
Transgenic product class
gain_of_function_variant
(Boiko et al., 2012)
missense_variant
(Boiko et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
gain_of_function_variant
(Boiko et al., 2012)
missense_variant
(Boiko et al., 2012)
Mutagen
in vitro construct
(Boiko et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-ppk.deg.FLAG}
Cytology
Description

UASt regulatory sequences drive expression of a gain-of-function mutant of ppk (contains the amino acid replacement S551F), tagged at the C-terminal end with Tag:FLAG.

(Boiko et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
ppk
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The amplitude of the amiloride-sensitive leak current and of the acid-evoked transient inward Na[+] current (I[[t]]) is increased compared to wild type in cultured class IV multidendritic neurons derived from animals expressing ppkdeg.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ppk.1.9. In addition, the inactivation of I[[t]] is slowed.

      Larvae expressing ppkdeg.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ppk.1.9 show defective locomotion (the degree of direction change per second is significantly reduced compared to wild type) and show a significant reduction in the response to a transient, noxious mechanical stimulus compared to wild type.

      (Boiko et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ppkdeg.Scer\UAS.T:Zzzz\FLAG
      ppkdeg.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)