FB2025_01 , released February 20, 2025
Allele: Dmel\RetUAS.Tag:FLAG,Tag:polyHis
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General Information
Symbol
Dmel\RetUAS.Tag:FLAG,Tag:polyHis
Species
D. melanogaster
Name
FlyBase ID
FBal0281733
Feature type
allele
Associated gene
Dmel\Ret
Associated Insertion(s)
Carried in Construct
P{UAS-Ret.3xFLAG-6xHis}
Key Links
Transgenic product class
wild_type
(Kallijärvi et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kallijärvi et al., 2012)
Mutagen
in vitro construct
(Kallijärvi et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-Ret.3xFLAG-6xHis}
Cytology
Description

UASt regulatory sequences drive expression of Ret which is tagged at the C-terminal end with Tag:FLAG and with Tag:polyHis.

(Kallijärvi et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Ret
Tagged with
Tag:FLAG
Tag:polyHis
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult flies expressing RetScer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 driven by Scer\GAL4esg-NP7397 combined with tub-Gal80[ts] (for temporal control) or flies expressing the transgene in MARCM clones under the control of Scer\GAL4tub.PU display significant increase in midgut stem cell proliferation (assessed by number of pH3-positive cells). Scer\GAL4wg.KO-driven expression fails to promote intestinal stem cell proliferation.

      (Perea et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing RetScer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 driven by Scer\GAL4esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is suppressed by co-expression of any of the following: panΔN.Scer\UAS, Src42AKK108017 or FakScer\UAS.T:Ivir\HA1 but not Src64BGD12263.

      (Perea et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      RetUAS.Tag:FLAG,Tag:polyHis/Scer\GAL4Ret.P2 partially rescues RetLM1

      (Myers et al., 2018)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      RetScer\UAS.T:Zzzz\FLAG,T:Zzzz\His6
      RetUAS.Tag:FLAG,Tag:polyHis
      Name Synonyms
      Secondary FlyBase IDs
        References (3)