FlyBase Allele Report: Hsap\YWHAG[hs.cHa]

General Information

Symbol

Hsap\YWHAG^hs.cHa

Species

H. sapiens

Name

Heat shock construct a of Hong

FlyBase ID

FBal0282029

Feature type

allele

Associated gene

Hsap\YWHAG

Associated Insertion(s)

Carried in Construct

P{hs-YWHAG.H}

Key Links

Transgenic product class

cDNA

(Hong et al., 2008)

wild_type

(Hong et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Hong et al., 2008)

wild_type

(Hong et al., 2008)

Mutagen

in vitro construct

(Hong et al., 2008)

Progenitor genotype

Carried in construct

P{hs-YWHAG.H}

Cytology

Description

Hsp70 regulatory sequences drive expression of a Hsap\YWHAG cDNA.

(Hong et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Hsap\YWHAG

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | third instar larval stage, with Scer\GAL4^GMR.PF

(Hong et al., 2008)

visible | adult stage | heat sensitive

(Hong et al., 2008)

Phenotype Manifest In

eye | heat sensitive

(Hong et al., 2008)

eye disc | third instar larval stage

(Hong et al., 2008)

Detailed Description

Statement

Reference

1.61% of flies expressing Hsap\YWHAG^hs.cHa have a rough eye phenotype when heat-shocked at 37[o]C for 30 minutes every 7.5 hours from the third instar larval stage.

An increased number of elav-positive proneural eye disc cells are going through S-phase (visualised using BrdU) in heat-shocked third instar larvae expressing 3 or 4 copies of Hsap\YWHAG^hs.cHa (homozygous/heterozygous for an X chromosome insertion and homozygous for a 3rd chromosome insertion) in comparison with non-heat-shocked controls.

(Hong et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\YWHAG^hs.cHa has visible | adult stage | heat sensitive phenotype, enhanceable by stg⁶/stg[+]

(Hong et al., 2008)

Phenotype Manifest In

Other

Statement

Reference

Hsap\YWHAG^hs.cHa, stg⁶/stg[+] has eye | heat sensitive phenotype

(Hong et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Heterozygous stg⁶ induces a rough eye phenotype when flies expressing Hsap\YWHAG^hs.cHa are heat-shocked at 37[o]C for 30 minutes every 7.5 hours from the third instar stage. 8.96% of flies have a rough eye compared to only 1.61% in the absence of stg⁶ and 1.39% in wild type controls. No eye defects are seen in heterozygous stg⁶.

(Hong et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\YWHAG^hs.cHa

Name Synonyms

Heat shock construct a of Hong

Secondary FlyBase IDs

References (1)

Report Sections

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