FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Avic\GFPEGFP.E(spl)mα-BFM.1.0.Tag:NLS(tra)
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General Information
Symbol
Avic\GFPEGFP.E(spl)mα-BFM.1.0.Tag:NLS(tra)
Species
A. victoria
Name
FlyBase ID
FBal0282415
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
P{E(spl)mα-BFM-GFP.1.0.L}
P{E(spl)mα-BFM-GFP.1.0}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Castro et al., 2005, Barolo et al., 2004)
Progenitor genotype
Carried in construct
P{E(spl)mα-BFM-GFP.1.0.L}
P{E(spl)mα-BFM-GFP.1.0}
Cytology
Description

A 1.0kb genomic fragment from the E(spl)mα-BFM gene (nucleotides -71 to -1083) is fused upstream of a minimal Hsp70 promoter driving expression of EGFP tagged at the C-terminal end with a nuclear localization signal (Tag:NLS(tra)).

(Barolo et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
E(spl)mα-BFM
Encoded product / tool
EGFP
Tagged with
Tag:NLS(tra)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage -- pupal stage
      proneural cluster | restricted
      (Liu and Posakony, 2012)
      prepupal stage P3 -- P4
      anterior-posterior compartment boundary of the wing disc

      Comment: anterior side

      (Casso et al., 2011)
      Additional Information
      Statement
      Reference

      AvicGFPEGFP.E(spl)mα-BFM.1.0.Tag:NLS(tra) is expressed in the non-SOP cells of proneural clusters at larval and pupal stages.

      (Liu and Posakony, 2012)

      Reporter expression is observed in pupal wing discs near the anterior posterior (AP) border on the anterior side of the border.

      (Casso et al., 2011)
       
      Marker for
      Reflects expression of
      E(spl)mα-BFM
      (Liu and Posakony, 2012, Casso et al., 2011)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Avic\GFPE(spl)mα-BFM.1.0.T:nls-tra
      Avic\GFPEGFP.E(spl)mα-BFM.1.0.Tag:NLS(tra)
      Avic\GFPmα.H-Stinger
      Avic\GFPmα.hs.T:nls-tra
      Avic\GFPmα.hs.T:nls5
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0190207
      • FBal0157324
      References (5)