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General Information
Symbol
Ivir\M2H37A.UAS
Species
O. Influenza virus
Name
FlyBase ID
FBal0282759
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a H37A mutant of Ivir\M2.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Ivir\M2H37A.Scer\UAS under the control of Scer\GAL4ey.PH results in the lack of eclosion of flies. When flies are removed from their pupal casings, they are found arrested at different developmental stages ranging from early pupae to almost fully developed flies. As a result, some cephalic structures consist of only the presumptive proboscis still attached to the larval mouth hook, while others are fully formed though lacking any eyes. Transgenic lines expressing Ivir\M2H37A.Scer\UAS at lower level verify that the toxin is working specifically to ablate the eyes. A small number of flies from these crosses are able to eclose and they display a total absence of eye tissue, reduced eyes, and/or cyclopia.

Expression of Ivir\M2H37A.Scer\UAS under the control of Scer\GAL4msn.PL results in the ablation of almost all lamellocytes. Those lamellocytes that remain are aggregated into large melanotic masses in third instar larvae.

Expression of Ivir\M2H37A.Scer\UAS under the control of Scer\GAL4He.PZ results in a significant reduction in hemocyte cells, including ablation of the salivary glands in all third instar larvae and melanotic masses in the hemolymph that attached to organelles in roughly half of the total population screened.

Scer\GAL4HCH.Hand>Ivir\M2H37A.Scer\UAS induced cell death does not commence until mid to late second instar larval stage. Scer\GAL4HCH.Hand>Ivir\M2H37A.Scer\UAS-expressing adult flies display zero to one pericardial cell associated with the dorsal vessel. Expression of Scer\GAL4HCH.Hand>Ivir\M2H37A.Scer\UAS results in the specific ablation of excretory cells (pericardiac nephrocytes) that flank the dorsal vessel. These flies die prematurely compared with controls.

Analysis of the phase of lethality resulting from the expression of Ivir\M2H37A.Scer\UAS under the control of Scer\GAL4HCH.Hand indicates no significant death (as compared with controls) during embryogenesis, larval or pupal stages.

No ablation of Scer\GAL4elav-C155>Ivir\M2H37A.Scer\UAS-expressing nervous system cells is observed during embryonic stages. Ablation of cells only begins to be apparent during the middle of the second instar larval stage.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Ivir\M2H37A.Scer\UAS expresses a toxic ion channel protein that can be used in combination with a Scer\GAL4 driver for specific cell ablation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Ivir\M2H37A.Scer\UAS
Ivir\M2H37A.UAS
Name Synonyms
Secondary FlyBase IDs
    References (1)