FB2026_02 , released June 18, 2026
Allele: Dmel\fra::robo1SD.UAS.Tag:V5,Tag:polyHis,Tag:HA,Tag:SS(wg)
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General Information
Symbol
Dmel\fra::robo1SD.UAS.Tag:V5,Tag:polyHis,Tag:HA,Tag:SS(wg)
Species
D. melanogaster
Name
FlyBase ID
FBal0283330
Feature type
allele
Associated gene
fra::robo1
Associated Insertion(s)
Carried in Construct
P{UAS-fra::robo1.SD}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Gilestro, 2008)
Progenitor genotype
Carried in construct
P{UAS-fra::robo1.SD}
Cytology
Description

UAS regulatory sequences drive expression of a mutant form of robo1 in which the region between amino acids 891 and 973 has been swapped with the analogous region of fra. The construct has a signal sequence (Tag:SS(wg)) followed by three Tag:HA tags at the N-terminal end and the Tag:V5 epitope and Tag:polyHis tag at the C-terminal end.

(Gilestro, 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
fra::robo1
Tagged with
Tag:HA
Tag:polyHis
Tag:SS(wg)
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of fra::roboSD.Scer\UAS.T:SV5\V5,T:Zzzz\His6,T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4insc-Mz1407 almost completely rescues the ectopic commissural crossing phenotype seen in homozygous robo1 mutant embryos. Ectopic crossings are only seen in 20% of segments.

      When fra::roboSD.Scer\UAS.T:SV5\V5,T:Zzzz\His6,T:Ivir\HA1,T:SS-wg and commScer\UAS.T:Hsap\MYC are co-expressed under the control of Scer\GAL4insc-Mz1407 the axons collapse into the midline.

      (Gilestro, 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      fra::robo1SD.Scer\UAS.T:SV5\V5,T:Zzzz\His6,T:Ivir\HA1,T:SS-wg
      fra::robo1SD.UAS.Tag:V5,Tag:polyHis,Tag:HA,Tag:SS(wg)
      fra::roboSD.Scer\UAS.T:SV5\V5,T:Zzzz\His6,T:Ivir\HA1,T:SS-wg
      Name Synonyms
      Secondary FlyBase IDs
        References (1)