FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\LRRK2UAS.cLa.Tag:FLAG
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General Information
Symbol
Hsap\LRRK2UAS.cLa.Tag:FLAG
Species
H. sapiens
Name
FlyBase ID
FBal0284312
Feature type
allele
Associated gene
Hsap\LRRK2
Associated Insertion(s)
Carried in Construct
P{UAS-LRRK2.FLAG.L}
Also Known As
UAS-hLRRK2, UAS-Flag-LRRK2-WT
Key Links
Transgenic product class
wild_type
(Lin et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Lin et al., 2010)
Mutagen
in vitro construct
(Lin et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-LRRK2.FLAG.L}
Cytology
Description

UASt regulatory sequences drive expression of wild-type Hsap\LRRK2 tagged with Tag:FLAG at the N-terminus.

(Lin et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\LRRK2
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Hsap\LRRK2Scer\UAS.cLa.T:Zzzz\FLAG under the control of Scer\GAL4ple.PF, Scer\GAL4nSyb.PG, Scer\GAL4Gr5a.8.5, Scer\GAL4E49, Scer\GAL4Ddc.PL, Scer\GAL4Ddc.HL9, Scer\GAL4ple.TH-C' or Scer\GAL4ple.TH-D' leads to insignificant differences in proboscis extension reflex (in starved flies exposed to a moderate sugar stimulus), as compared to controls. In ad libitum conditions, Scer\GAL4ple.PF-driven expression leads to a significant decrease in lifespan, as compared to controls.

      (Cording et al., 2017)

      As in controls, the dynamic golgi outposts (GOPs) in the dendrites of dendritic arborizing neurons expressing Hsap\LRRK2Scer\UAS.cLa.T:Zzzz\FLAG under the control of Scer\GAL4ppk.PU show an overall preference for retrograde movement (towards cell bodies). However, anterograde movement (towards dendritic ends) is slightly increased compared to controls and retrograde displacement is reduced. Velocity of movement is unchanged in both directions.

      (Lin et al., 2015)

      Flies expressing Hsap\LRRK2Scer\UAS.cLa.T:Zzzz\FLAG under the control of Scer\GAL4ple.PG do not show a progressive reduction in electroretinogram (ERG) amplitude (there is no significant difference in the amplitude seen in 3 and 28 day old flies).

      (Hindle et al., 2013)

      Expression of Hsap\LRRK2Scer\UAS.cLa.T:Zzzz\FLAG in dendritic arborization (DA) neurons using Scer\GAL4109(2)80 engenders dendrite arborization defects as shown by the quantification of numbers of dendritic ends in the dorsal field of A6 segments in third instar larvae.

      Axonal terminals of class IV dendritic arborizing neurons expressing Scer\GAL4ppk.PG>Hsap\LRRK2Scer\UAS.cLa.T:Zzzz\FLAG remain normal, targeting to the ventral nerve cord, although the complexity of dendrites is severely compromised, displaying shorter and fewer terminal arbors and leaving some receptive fields uncovered.

      (Lin et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\LRRK2Scer\UAS.cLa.T:Zzzz\FLAG in dendritic arborizing neurons under the control of either Scer\GAL4109(2)80 or Scer\GAL4ppk.PU suppresses the dendrite overgrowth seen in Df(3R)del6/Lrrke03680 mutants.

      Expression of Hsap\LRRK2Scer\UAS.cLa.T:Zzzz\FLAG in dendritic arborizing class IV neurons under the control of Scer\GAL4ppk.PU suppresses the preference for anterograde movement (towards dendritic ends) seen in Df(3R)del6/Lrrke03680 mutant golgi outposts (GOPs), with the resulting GOPs showing the retrograde preference (towards cell bodies) seen in the Scer\GAL4ppk.PU control.

      (Lin et al., 2015)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\LRRK2Scer\UAS.cLa.T:Zzzz\FLAG
      Hsap\LRRK2UAS.cLa.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (7)