FlyBase Allele Report: Mmus\Prnp[WT.UAS]

General Information

Symbol

Mmus\Prnp^WT.UAS

Species

M. musculus

Name

FlyBase ID

FBal0284332

Feature type

allele

Associated gene

Mmus\Prnp

Associated Insertion(s)

Carried in Construct

P{UAS-Prp.WT}

Transgenic product class

wild_type

(Deleault et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Deleault et al., 2003)

Mutagen

in vitro construct

(Deleault et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Prp.WT}

Cytology

Description

UASt drives expression of a 765bp fragment containing the wild type Mmus\Prnp open reading frame.

(Deleault et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Prnp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of prion disease

CEA

(Sanchez-Garcia et al., 2016, Sanchez-Garcia et al., 2013, Fernandez-Funez et al., 2010, Fernandez-Funez et al., 2009)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^BG380

(Fernandez-Funez et al., 2010)

abnormal locomotor behavior | adult stage, with Scer\GAL4^BG380

(Sanchez-Garcia et al., 2013)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^BG380

(Sanchez-Garcia et al., 2016, Fernandez-Funez et al., 2009)

abnormal neuroanatomy | adult stage, with Mmus\Prnp^{M205S.M212S.UAS}, Scer\GAL4^ey-OK107

(Sanchez-Garcia et al., 2013)

abnormal neuroanatomy | adult stage, with Mmus\Prnp^M205S.UAS, Scer\GAL4^ey-OK107

(Sanchez-Garcia et al., 2013)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Sanchez-Garcia et al., 2013)

abnormal neuroanatomy | progressive, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2010)

abnormal neuroanatomy | progressive, with Scer\GAL4^ey-OK107

(Fernandez-Funez et al., 2010)

short lived, with Scer\GAL4^da.G32

(Sanchez-Garcia et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Mmus\Prnp^WT.Scer\UAS under the control of Scer\GAL4^BG380 results in severe, progressive defects in climbing performance, as compared to controls.

(Sanchez-Garcia et al., 2016)

Expression of Mmus\Prnp^WT.Scer\UAS under the control of Scer\GAL4^da.G32 leads to a reduced adult lifespan compared to controls.

(Sanchez-Garcia et al., 2013)

The brains of 30-day-old flies expressing Scer\GAL4^da.G32>Mmus\Prnp^WT.Scer\UAS accumulate vacuoles mostly in the optic lobes. In these flies, the cortex contains few micro-vacuoles.

Flies older than 40 days expressing Scer\GAL4^ey-OK107>Mmus\Prnp^WT.Scer\UAS display completely normal morphology of the mushroom body lobes alpha, beta and gamma.

Older flies expressing Mmus\Prnp^WT.Scer\UAS under the control of Scer\GAL4^ey-OK107 show a modest but significant reduction in the size of Kenyon cell clusters size compared with controls.

Flies expressing Mmus\Prnp^WT.Scer\UAS under the control of Scer\GAL4^BG380 show severe locomotor dysfunction by the age of 4 days. 20-day-old flies expressing Scer\GAL4^BG380>Mmus\Prnp^WT.Scer\UAS show a moderate but significant decrease in activity levels compared with controls. The walking speed of flies expressing Scer\GAL4^BG380>Mmus\Prnp^WT.Scer\UAS show a modest but significant reduction compared with controls.

(Fernandez-Funez et al., 2010)

Scer\GAL4^BG380-mediated expression of Mmus\Prnp^WT.Scer\UAS triggers a severe locomotor dysfunction (decreased performance in climbing assay) in a progressive manner.

(Fernandez-Funez et al., 2009)

Scer\GAL4^Cha.7.4-mediated expression of Mmus\Prnp^WT.Scer\UAS does not cause an abnormal phenotype.

Scer\GAL4^Cha.7.4 Mmus\Prnp^WT.Scer\UAS third instar larval brains display no obvious neuropathology.

Flies expressing Mmus\Prnp^WT.Scer\UAS under the control of Scer\GAL4^Cha.7.4 or Scer\GAL4^Ddc.PL perform normally in a climbing assay and have normal adult longevity.

The adult brains of aged Scer\GAL4^Cha.7.4 Mmus\Prnp^WT.Scer\UAS files show only infrequent neuropil vacuoles.

(Gavin et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mmus\Prnp^WT.Scer\UAS

Mmus\Prnp^WT.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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