FlyBase Allele Report: Dmel\Alk[ts]

General Information

Symbol

Dmel\Alk^ts

Species

D. melanogaster

Name

FlyBase ID

FBal0284378

Feature type

allele

Associated gene

Dmel\Alk

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

heat sensitive hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

heat sensitive hypomorphic allele - genetic evidence

(Rohrbough et al., 2013)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: N1245D.

(Rohrbough et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | third instar larval stage

(Rohrbough et al., 2013)

abnormal neurophysiology | third instar larval stage (with Alk^f01491)

(Rohrbough et al., 2013)

abnormal sleep (with Alk¹)

(Bai and Sehgal, 2015)

abnormal sleep (with Df(2R)Exel7144)

(Bai and Sehgal, 2015)

abnormal sleep | :heat sensitive | conditional

(Bai and Sehgal, 2015)

abnormal sleep | heat sensitive (with Alk¹)

(Bai and Sehgal, 2015)

abnormal sleep | heat sensitive (with Df(2R)Exel7144)

(Bai and Sehgal, 2015)

abnormal sleep | recessive | heat sensitive

(Bai and Sehgal, 2015)

lethal | heat sensitive

(Rohrbough et al., 2013)

lethal | heat sensitive (with Alk¹)

(Rohrbough et al., 2013)

Phenotype Manifest In

midgut

(Rohrbough et al., 2013)

Detailed Description

Statement

Reference

Alk^ts/Alk¹ flies raised at 18[o] do not have significant changes in body length.

When sleep is measured using single beam monitors at the permissive temperature (18[o]C), Alk^ts/Alk¹ and Alk^ts/Df(2R)Exel7144 females have a mild but significant increase in total sleep compared to controls (no significant changes in males); acute inhibition of Alk at the restrictive temperature (29[o]C) in Alk^ts/Alk^ts, Alk^ts/Alk¹ and Alk^ts/Df(2R)Exel7144 flies leads to significant increases in sleep amount compared to controls (no effect in Alk^ts/+ females, a small but significant increase in males).

When sleep is measured using multi-beam monitors at 18[o]C, Alk^ts/Alk¹ flies have similar total sleep amount as controls, but at 29[o]C show significant total sleep increases (both day and night). At 29[o]C both Alk^ts/Alk¹ and Alk¹/+ females have significant decreases in waking activity and show normal geotaxis activity. Response to a mechanical stimulus during sleep (arousability) is similar in control and Alk^ts/Alk^ts females (at ZT6 and ZT20), though mutants are significantly less arousable at ZT22.

At 29[o]C, homeostatic sleep recovery after 6hr mechanical sleep deprivation in Alk^ts/Alk^ts females is similar to controls apart from a significant decrease in sleep latency.

Alk^ts/Alk^ts and Alk^ts/Alk¹ and Alk^ts/Df(2R)Exel7144 raised at 18[o]C maintain locomotor rhythms at 29[o]C in darkness; Alk^ts/Alk^ts flies raised at 25[o]C also does not cause arrhythmia.

(Bai and Sehgal, 2015)

Alk^ts fails to complement Alk¹ lethality at 29[o]C and shows a mutant midgut phenotype. Alk^ts animals show limited adult viability at 25[o]C, while Alk¹/Alk^ts animals show larval lethality.

Alk^ts and Alk¹/Alk^ts animals can be effectively maintained to third instar or pupal stages by initial rearing at 18[o]C for 48-72 hours from egg laying, followed by subsequent rearing at 25[o]C to the third larval instar. Excitatory junctional current (EJC) amplitude is increased by 79% compared to controls in Alk^ts third instar larvae and is increased by 210% in Alk¹/Alk^ts third instar larvae raised under these conditions.

Alk^ts/Alk^f01491 animals reared for 48 hours at 18[o]C and then subsequently shifted to 25[o]C show a strong increase (2.4-fold) in the mean excitatory junctional current (EJC) amplitude compared to controls.

(Rohrbough et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Alk^ts/Alk¹ is a non-suppressor of abnormal locomotor rhythm phenotype of Nf1^P2/Nf1^P1

(Bai and Sehgal, 2015)

Other

Statement

Reference

Alk^ts/Alk¹, Nf1^P2/Nf1^P1 has abnormal sleep phenotype

(Bai and Sehgal, 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Nf1^P1/Nf1^P2 mutations suppress (to different degrees) the long sleep phenotype seen in Alk^ts/Alk¹ males and females at different temperatures (18, 25 or 29[o]C). In male flies, Alk^ts/Alk¹;Nf1^P1/Nf1^P2 double mutants even sleep significantly less than wild type flies.

Alk^ts/Alk¹ does not suppress arrhythmicity (in dark conditions) in Nf1^P1/Nf1^P2 flies.

(Bai and Sehgal, 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Alk^ts is rescued by Alk^UAS.cLa/Scer\GAL4^386Y

(Bai and Sehgal, 2015)

Alk^ts is rescued by Alk^UAS.cLa/Scer\GAL4¹⁴⁷¹

(Bai and Sehgal, 2015)

Partially rescued by

Alk^ts is partially rescued by Alk^UAS.cLa/Scer\GAL4^30Y

(Bai and Sehgal, 2015)

Alk^ts is partially rescued by Scer\GAL4^121Y/Alk^UAS.cLa

(Bai and Sehgal, 2015)

Not rescued by

Alk^ts is not rescued by Scer\GAL4^007Y/Alk^UAS.cLa

(Bai and Sehgal, 2015)

Alk^ts is not rescued by Scer\GAL4^MJ63/Alk^UAS.cLa

(Bai and Sehgal, 2015)

Comments

Alk^ts fails to complement Alk¹ lethality at 29[o]C and fully complements Alk¹ at 18[o]C. At 25[o]c, Alk¹/Alk^ts animals show larval lethality.

Overexpression of Alk from the Scer\UAS regulatory sequences in the PBac{WH}Alk^f01491 insertion rescues the increased mean excitatory junctional current amplitude seen in Alk^ts/Alk^f01491 animals reared for 48 hours at 18[o]C and then subsequently shifted to 25[o]C.

(Rohrbough et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Alk^ts

(Bai and Sehgal, 2015)

alk^ts

(Rohrbough et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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