FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Mon1mut4
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General Information
Symbol
Dmel\Mon1mut4
Species
D. melanogaster
Name
FlyBase ID
FBal0285007
Feature type
allele
Associated gene
Dmel\Mon1
Associated Insertion(s)
Carried in Construct
Also Known As
Dmon1mut4
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
    Nature of the Allele
    Allele class

    amorphic allele - genetic evidence

    (Yousefian et al., 2013)
    Mutagen
    Progenitor genotype
    Cytology
    Description

    Nucleotide substitution: C?T.

    (Yousefian et al., 2013)

    Amino acid replacement: Q70term.

    (Yousefian et al., 2013)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    2L:4,850,958..4,850,958 [+]
    Nucleotide change:

    C4850958T

    Reported nucleotide change:

    C?T

    Amino acid change:

    Q70term | Mon1-PA

    Reported amino acid change:

    Q70term

    (Yousefian et al., 2013)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        The number of acidic autolysosomes (detected by LysoTracker staining) is strongly reduced and the number of autophagy structures is increased in fat body cells of starved Mon1mut4/Df(2L)ED7853 mutant third instar larvae relative to controls, while the cells show abnormal accumulation of autophagosomes. Similarly, cells in Mon1mut4 mutant somatic clones in larval fat body display fewer acidic autolysosomes and more autophagosomes than the surrounding control cells.

        (Hegedűs et al., 2016)

        Cells in homozygous clones in the wing disc contain enlarged endosomes which are strongly acidified. The number of cells in a given area is increased in the mutant disc compared to wild type.

        (Yousefian et al., 2013)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        NOT Suppressor of
        Statement
        Reference

        Mon1mut4/Mon1mut4 is a non-suppressor of abnormal cell size | somatic clone | third instar larval stage phenotype of Rab52

        (Hegedűs et al., 2016)
        Phenotype Manifest In
        NOT suppressed by
        NOT Suppressor of
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Somatic clones of fat body cell double mutant for both Rab52 and Mon1mut4 are larger than their neighboring control cells (as are Rab52 single mutant cells) but display similar abnormal accumulation of autophagosomes and lack of acidic autolysosomes as Mon1mut4 mutant cells in starved third instar larvae.

        (Hegedűs et al., 2016)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        Mon1mut4/Df(2L)ED7853 is rescued by Mon1UAS.Tag:HA/Scer\GAL4hs.PU

        (Hegedűs et al., 2016)

        Mon1mut4 is rescued by Scer\GAL4hh-Gal4/Mon1UAS.cYa

        (Yousefian et al., 2013)

        Mon1mut4 is rescued by Mon1UAS.Tag:HA/Scer\GAL4hh-Gal4

        (Yousefian et al., 2013)
        Comments

        Expression of Mon1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hs.PU rescues the loss of acidic autolysosomes (detected by LysoTracker staining) observed in fat cells of starved Mon1mut4/Df(2L)ED7853 third instar larvae.

        (Hegedűs et al., 2016)

        Expression of either Mon1Scer\UAS.cYa or Mon1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hh-Gal4 rescues the enlarged endosome phenotype of Mon1mut4 cells.

        (Yousefian et al., 2013)
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer

        Separable from: bib1.

        (Yousefian et al., 2013)

        The Mon1mut4 allele is a background mutation present on some bib1 chromosomes, and it accounts for the aberrant protein trafficking phenotype that was erroneously attributed to bib1 in FBrf0204892.

        (Yousefian et al., 2013)
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (4)
        Reported As
        Symbol Synonym
        Dmon1Mut4
        (Pannen et al., 2020)
        Dmon1mut4
        (Troost et al., 2023, Yousefian et al., 2013)
        Mon1d4
        (Hegedűs et al., 2016)
        Mon1mut4
        Name Synonyms
        Secondary FlyBase IDs
          References (4)