FB2026_02 , released June 18, 2026
Allele: Hsap\LRRK2G2019S.UAS.Tag:V5
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General Information
Symbol
Hsap\LRRK2G2019S.UAS.Tag:V5
Species
H. sapiens
Name
FlyBase ID
FBal0285581
Feature type
allele
Associated gene
Hsap\LRRK2
Associated Insertion(s)
Carried in Construct
PBac{UAS-hLRRK2.G2019S}
Also Known As
LRRK2G2019S
Key Links
Transgenic product class
missense_variant
(Matta et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Matta et al., 2012)
Mutagen
in vitro construct
(Matta et al., 2012)
Progenitor genotype
Carried in construct
PBac{UAS-hLRRK2.G2019S}
Cytology
Description

UASt regulatory sequences drive expression of a kinase hyperactive clinical mutant form of Hsap\LRRK2 (contains the amino acid replacement G2019S) which is tagged with Tag:V5.

(Matta et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\LRRK2
Tagged with
Tag:V5
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Matta et al., 2012)
      LRRK2:p.Gly2019Ser
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: LRRK2_1940
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Hsap\LRRK2G2019S.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4Toll-6-D42 results in the presynaptic region of third instar larval neuromuscular junctions exhibiting a significant increase in the density of autophagosomes (detected as fluorescent puncta of Atg8 or Lamp in confocal microscopy images) under normal diet, but exhibiting no defects in starvation-induced formation of autophagosomes, as compared to controls.

      (Soukup et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4da.G32/Hsap\LRRK2G2019S.UAS.Tag:V5 is a non-suppressor of autophagosome | third instar larval stage | nutrition conditional phenotype of EndoA26/EndoAΔ4, EndoAS75A.CyPet

      (Soukup et al., 2016)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The expression of Hsap\LRRK2G2019S.Scer\UAS.T:SV5\V5 under the control of Scer\GAL4Toll-6-D42 does not rescue the defective starvation-induced formation of autophagosomes observed at the presynaptic region of the third instar larval neuromuscular junction of EndoAΔ4/EndoA26 transheterozygotes that also possess one copy of EndoAS75A.T:Avic\GFP-CyPet.

      (Soukup et al., 2016)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\LRRK2G2019S.Scer\UAS.T:SV5\V5
      Hsap\LRRK2G2019S.UAS.Tag:V5
      LRRK2G2019S
      (Soukup et al., 2016, Matta et al., 2012)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)