The FRT cassette has been excised, allowing the Act5C promoter to drive expression of pk that is tagged at the N-terminal with Tag:MYC and has a Tag:CS(PSP) cleavage site followed by a Tag:HA tag within a non-conserved region near its C-terminus.
pkAct5C.Tag:MYC,Tag:HA,Tag:CS(PSP) rescues pkpk-sple-13
Expression of pkAct5C.T:Hsap\MYC,T:Ivir\HA1,T:Zzzz\PSP.CS fully rescues the wing planar polarity phenotype seen in pkpk-sple-13 mutant wings.