UAS regulatory sequences drive expression of a mutant form of Smox in which G450, Y455, R457 and Q458 have all been replaced by alanines. The construct is tagged with Tag:FLAG.
SmoxRB4.UAS.Tag:FLAG/Scer\GAL4da.G32 fails to rescue SmoxF4
Expression of SmoxRB4.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 fails to rescue the wing disc widening phenotype seen in SmoxF4 mutant third instar larvae.