FlyBase Allele Report: Dmel\eya[WT.UAS.Tag:NLS(SV40-largeT)]

General Information

Symbol

Dmel\eya^{WT.UAS.Tag:NLS(SV40-largeT)}

Species

D. melanogaster

Name

FlyBase ID

FBal0287990

Feature type

allele

Associated gene

Dmel\eya

Associated Insertion(s)

Carried in Construct

P{UAS-eya.WT.NLS}

Key Links

Transgenic product class

wild_type

(Xiong et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Xiong et al., 2009)

Mutagen

in vitro construct

(Xiong et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-eya.WT.NLS}

Cytology

Description

UAS regulates expression of wild type eya fused to a Tag:NLS(SV40-largeT) nuclear localization sequence.

(Xiong et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

eya

Tagged with

Tag:NLS(SV40-largeT)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye | ectopic, with Scer\GAL4^dpp.blk1

(Xiong et al., 2009)

eye | ectopic, with Scer\GAL4^dpp.blk1, eya^{K699Q.UAS.Tag:Myr(v-Src)}

(Xiong et al., 2009)

eye | ectopic, with Scer\GAL4^dpp.blk1, eya^{WT.UAS.Tag:Myr(v-Src)}

(Xiong et al., 2009)

Detailed Description

Statement

Reference

Scer\GAL4^dpp.blk1 eya^{WT.Scer\UAS.T:SV40\nls2} flies exhibit an average of 20% frequency of ectopic eye induction (compared to 49% frequency when eya^WT.Scer\UAS is expressed).

Co-expression of eya^{WT.Scer\UAS.T:SV40\nls2} and eya^{WT.Scer\UAS.T:Uuuu\Myr4} via Scer\GAL4^dpp.blk1 results in approximately two-fold more ectopic eye induction relative to expression of eya^{WT.Scer\UAS.T:SV40\nls2} alone.

Co-expression of eya^{WT.Scer\UAS.T:SV40\nls2} and eya^{K699Q.Scer\UAS.T:Uuuu\Myr4} via Scer\GAL4^dpp.blk1 results in a limited increase (~10%) in ectopic eye induction relative to expression of eya^{WT.Scer\UAS.T:SV40\nls2} alone.

(Xiong et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

eya^{WT.UAS.Tag:NLS(SV40-largeT)}/Scer\GAL4^dpp.blk1 partially rescues eya²

(Xiong et al., 2009)

eya^{WT.UAS.Tag:NLS(SV40-largeT)}, Scer\GAL4^dpp.blk1, eya^{WT.UAS.Tag:Myr(v-Src)} partially rescues eya²

(Xiong et al., 2009)

eya^{WT.UAS.Tag:NLS(SV40-largeT)}, Scer\GAL4^dpp.blk1, eya^{K699Q.UAS.Tag:Myr(v-Src)} partially rescues eya²

(Xiong et al., 2009)

Comments

Scer\GAL4^dpp.blk1-mediated expression of eya^{WT.Scer\UAS.T:SV40\nls2} rescues the eyeless phenotype of eya² animals at 48% efficiency, compared to the 100% rescue efficiency of eya^WT.Scer\UAS.

Co-expression of eya^{WT.Scer\UAS.T:SV40\nls2} and eya^{WT.Scer\UAS.T:Uuuu\Myr4} via Scer\GAL4^dpp.blk1 results in a more marked rescue of the eyeless phenotype of eya² animals relative to expression of eya^{WT.Scer\UAS.T:SV40\nls2} alone.

Co-expression of eya^{WT.Scer\UAS.T:SV40\nls2} and eya^{K699Q.Scer\UAS.T:Uuuu\Myr4} via Scer\GAL4^dpp.blk1 results in a greater rescue of the eyeless phenotype of eya² animals relative to expression of eya^{WT.Scer\UAS.T:SV40\nls2} alone, though not as great a rescue as when eya^{WT.Scer\UAS.T:Uuuu\Myr4} is co-expressed.

(Xiong et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

eya^{WT.Scer\UAS.T:SV40\nls-largeT}

eya^{WT.Scer\UAS.T:SV40\nls2}

eya^{WT.UAS.Tag:NLS(SV40-largeT)}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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