FlyBase Allele Report: Dmel\eIF3m[E]

General Information

Symbol

Dmel\eIF3m^E

Species

D. melanogaster

Name

FlyBase ID

FBal0288455

Feature type

allele

Associated gene

Dmel\eIF3m

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(D'Brot et al., 2013)

amorphic allele - molecular evidence

(D'Brot et al., 2013)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: Q135term.

(D'Brot et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:14,163,959..14,163,959 [-]

Nucleotide change:

C14163959T

Amino acid change:

Q135term | eIF3m-PA; Q135term | eIF3m-PB

Reported amino acid change:

Q135term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(D'Brot et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of first instar larval stage

(D'Brot et al., 2013)

lethal - all die before end of first instar larval stage (with Df(2R)Exel7130)

(D'Brot et al., 2013)

male sterile (with eIF3m^L)

(D'Brot et al., 2013)

partially lethal - majority live (with eIF3m^L)

(D'Brot et al., 2013)

short lived (with eIF3m^L)

(D'Brot et al., 2013)

visible | adult stage (with eIF3m^L)

(D'Brot et al., 2013)

Phenotype Manifest In

cystic bulge (with eIF3m^L)

(D'Brot et al., 2013)

investment cone (with eIF3m^L)

(D'Brot et al., 2013)

wing (with eIF3m^L)

(D'Brot et al., 2013)

Detailed Description

Statement

Reference

33% of Tango7^L/Tango7^E mutant flies have wrinkled wings. Most flies die during eclosion or soon thereafter. Mild wing blemishing and upward curved wings are seen at low penetrance.

The investment cones in Tango7^L/Tango7^E mutant spermatid cysts are able to form properly, but either stall at the nuclei or move asynchronously through the synctia.

(D'Brot et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

eIF3m^E/eIF3m^L is rescued by eIF3m^+tVK006

(D'Brot et al., 2013)

eIF3m^E is rescued by eIF3m^+tVK006

(D'Brot et al., 2013)

Comments

Expression of Tango7^+tVK006 rescues the lethality seen in homozygous Tango7^E mutants. Spermatid individualisation also occurs correctly in these flies.

Expression of Tango7^+tVK006 rescues the semi-viability, male sterility and wrinkled wing phenotypes seen in Tango7^L/Tango7^E mutants.

(D'Brot et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tango7^E

(D'Brot et al., 2013)

eIF3m^E

tango7^E

(D'Brot et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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