FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Ubc6Δ1
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General Information
Symbol
Dmel\Ubc6Δ1
Species
D. melanogaster
Name
FlyBase ID
FBal0288690
Feature type
allele
Associated gene
Dmel\Ubc6
Associated Insertion(s)
P{3'EPgy2}Ubc6Δ1
Carried in Construct
Key Links
Allele class

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - molecular evidence

(Haddad et al., 2013)
Mutagen
P-element activity
(Haddad et al., 2013)
Progenitor genotype
P{EPgy2}Ubc6EY04634
(Haddad et al., 2013)
Associated Insertion(s)
P{3'EPgy2}Ubc6Δ1
Cytology
Description

The P{EPgy2} element in P{EPgy2}UbcD6EY04634 has been imprecisely excised. 8 nucleotides (GCCCAGGC, in wild type controls located between -50 and -58 nucleotides from the ATG start of dRad6) are duplicated and border a remaining piece of 42 nucleotides from the P-element (5’-CAT GAT GAA ATA ACA TTA ATG TTA AGA TGT TAT TTC ATC ATG-3’)

(Haddad et al., 2013)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  syndromic intellectual disability
      CEA
      (Haddad et al., 2013)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Defects in mitochondrial membrane potential are detected in the neuromuscular junctions of homozygous UbcD6Δ1 mutant third instar larvae.

      UbcD6Δ1 mutant larvae exhibit defects in synaptic vesicle trafficking. The levels of membrane uptake (dye internalisation) in UbcD6Δ1 mutant synaptic boutons in response to nerve stimulation is significantly reduced compared to controls. Synaptic transmission appears normal (both Excitatory Junctional Current (EJC) and miniature Excitatory Junctional Current (mEJC) are similar to controls). No effect is seen on Excitatory Junction Potential (EJP) at low frequencies, but at high frequencies EJP amplitudes in UbcD6Δ1 mutants are steadily reduced, indicating a reduced rate of vesicle replenishment. This reduction in EJP amplitude is less severe when motor neurons are filled with ATP.

      (Haddad et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Ubc6Δ1 is rescued by Ubc6+tHa

      (Haddad et al., 2013)
      Comments

      Expression of UbcD6+tHa rescues the lethality associated with homozygous UbcD6Δ1. The mitochondrial membrane potential and synaptic vesicle trafficking defects seen in UbcD6Δ1 mutant third instar larvae are also rescued, as are the defects in vesicle recycling (reduction in Excitatory Junctional Potential).

      (Haddad et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ubc6Δ1
      UbcD6Δ1
      dRad6Δ1
      (Haddad et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)