FlyBase Gene Report: Dmel\Ubc6

General Information

Symbol

Dmel\Ubc6

Species

D. melanogaster

Name

Ubiquitin conjugating enzyme 6

Annotation Symbol

CG2013

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004436

Gene Model Status

Current

Stock Availability

13 publicly available

Enzyme Name (EC)

E2 ubiquitin-conjugating enzyme (2.3.2.23)

Gene Summary

Ubiquitin conjugating enzyme 6 (Ubc6) encodes an enzyme that acts together with E3 ubiquitin ligases to transfer ubiquitin to target proteins. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

UbcD6, Rad6, Dhr6, dRad6

Key Links

Genomic Location

Cytogenetic map

82E3-82E4

Sequence location

3R:4,948,755..4,952,682 [-]

Recombination map

3-47.1

RefSeq locus

NT_033777 REGION:4948755..4952682

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (23 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables p53 binding

inferred from physical interaction with FLYBASE:p53; FB:FBgn0039044

(Chen et al., 2011)

enables protein domain specific binding

inferred from physical interaction with UniProtKB:Q8IMZ4

(Chen et al., 2011)

enables ubiquitin conjugating enzyme activity

inferred from mutant phenotype

(Cai et al., 2022)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ubiquitin conjugating enzyme activity

inferred by curator from GO:0006511

(Chen et al., 2011)

inferred from biological aspect of ancestor with PANTHER:PTN000630651

(GO Reference Genome Project, 2011-)

enables ubiquitin-protein transferase activity

inferred from sequence or structural similarity with SGD:S000003026

(Koken et al., 1991)

Biological Process (16 terms)

Terms Based on Experimental Evidence (15 terms)

CV Term

Evidence

References

involved_in centrosome cycle

inferred from mutant phenotype

(Goshima et al., 2007)

involved_in centrosome separation

inferred from mutant phenotype

(Dobbelaere et al., 2008)

involved_in DNA repair

inferred from genetic interaction with SGD:S000003026

(Koken et al., 1991)

involved_in endocytic recycling

inferred from mutant phenotype

(Haddad et al., 2013)

involved_in heart development

inferred from mutant phenotype

(Zhu et al., 2017)

involved_in negative regulation of apoptotic process

inferred from mutant phenotype

(Chen et al., 2011)

involved_in negative regulation of DNA damage response, signal transduction by p53 class mediator

inferred from mutant phenotype

(Chen et al., 2011)

involved_in negative regulation of ERK1 and ERK2 cascade

inferred from genetic interaction with FLYBASE:Usp47; FB:FBgn0016756

(Ashton-Beaucage et al., 2016)

involved_in negative regulation of heterochromatin formation

inferred from mutant phenotype

(Cai et al., 2022)

involved_in negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator

inferred from mutant phenotype

(Chen et al., 2011)

involved_in positive regulation of DNA-templated transcription

inferred from mutant phenotype

(Cai et al., 2022)

involved_in positive regulation of mitophagy

inferred from mutant phenotype

(Haddad et al., 2013)

involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Ashton-Beaucage et al., 2016)

inferred from mutant phenotype

(Chen et al., 2011)

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Chen et al., 2011)

involved_in ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Chen et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in DNA repair

inferred from biological aspect of ancestor with PANTHER:PTN000630651

(GO Reference Genome Project, 2011-)

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000630651

(GO Reference Genome Project, 2011-)

involved_in protein polyubiquitination

inferred from biological aspect of ancestor with PANTHER:PTN000630651

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Chen et al., 2011)

located_in nucleus

inferred from direct assay

(Chen et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of HULC complex

inferred from biological aspect of ancestor with PANTHER:PTN000630652

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

E2 UBIQUITIN CONJUGATING ENZYMES

Protein Family (UniProt)

Belongs to the ubiquitin-conjugating enzyme family. (P25153)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ubiquitin conjugating enzyme activity

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine (2.3.2.23)

Summaries

Gene Snapshot

Ubiquitin conjugating enzyme 6 (Ubc6) encodes an enzyme that acts together with E3 ubiquitin ligases to transfer ubiquitin to target proteins. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

E2 UBIQUITIN CONJUGATING ENZYMES -: Ubiquitin-conjugating (E2) enzymes accept activated ubiquitin from ubiquitin-activating (E1) enzymes via thioester bond with a conserved catalytic cysteine. E2s then interact with ubiquitin ligases (E3) to directly or indirectly ubiquitinate target proteins. E2s are characterised by a 200 amino acid ubiquitin-conjugating (UBC) domain which interacts with E1 and E3s and acts catalytically. (Adapted from PMID:19940261.)

Protein Function (UniProtKB)

E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase (By similarity). Required for postreplication repair of UV-damaged DNA (PubMed:1902572). Involved in the negative regulation of the Ras/MAPK signaling pathway in the wing by acting with the putative E3 ligases poe, Kcmf1 and Ufd4 to mediate the ubiquitination and proteasomal degradation of rl/MAPK (PubMed:27552662). Required for in mitophagy (PubMed:23685073).

(UniProt, P25153)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ubc6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P25153)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.39

Gene model reviewed during 5.53

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ubc6-RA

FBtr0078849

NM_079506

2166

151

Ubc6-RC

FBtr0306106

NM_001259987

1142

151

Additional Transcript Data and Comments

Reported size (kB)

2.1, 1.3 (northern blot)

(Koken et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ubc6-PA

FBpp0078490

17.2

151

5.78

FBpp0297243

17.2

151

5.78

P25153

NP_001246916

AFH06235

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

151 aa isoforms: Ubc6-PA, Ubc6-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

151 (aa); 17 (kD predicted)

(Koken et al., 1991)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ubc6 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ubc6_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.78

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Chen et al., 2011)

located_in nucleus

inferred from direct assay

(Chen et al., 2011)

Expression Deduced from Reporters

Reporter: P{ST.lexA::HG}Ubc6^SE101

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

larval central nervous system

(Bloomington Drosophila Stock Center, 2019)

larval ventral nerve cord

(Bloomington Drosophila Stock Center, 2019)

ring gland

(Bloomington Drosophila Stock Center, 2019)

larval enteroendocrine cell

(Bloomington Drosophila Stock Center, 2019)

eye disc

(Bloomington Drosophila Stock Center, 2019)

photoreceptor neuron

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval proventriculus

(Bloomington Drosophila Stock Center, 2019)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ubc6 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ubc6

View exonic expression by tissue for Dmel\Ubc6

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40610/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004436

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004436

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004436

Flygut - An atlas of the Drosophila adult midgut

FBgn0004436

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ubc6

Transgenic constructs containing regulatory region of Ubc6

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 11 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | pupal stage, with Scer\GAL4^Act5C.PI

Ubc6^RNAi.UAS

lethal - all die before end of larval stage | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Ubc6^GD17232

partially lethal - majority die, with Scer\GAL4^4XHand

Ubc6^RNAi.UAS.cUa

partially lethal - majority die | larval stage, with Scer\GAL4^Act5C.PI

Ubc6^RNAi.UAS

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ubc6^GD13294

viable, with Scer\GAL4^tin.CΔ4

Ubc6^GD13294

Other Phenotypes

Allele

abnormal neurophysiology | third instar larval stage

decreased cell number | adult stage, with Scer\GAL4^4XHand

Ubc6^RNAi.UAS.cUa

flightless, with Scer\GAL4^Mef2.PR

Ubc6^GD13294

increased cell death, with Scer\GAL4^en.PU

Ubc6^RNAi.UAS

short lived, with Scer\GAL4^4XHand

Ubc6^RNAi.UAS.cUa

some die during pupal stage, with Scer\GAL4^pnr-MD237

Phenotype manifest in

Allele

adult heart, with Scer\GAL4^4XHand

Ubc6^RNAi.UAS.cUa

cardiac myofibril | adult stage, with Scer\GAL4^4XHand

Ubc6^RNAi.UAS.cUa

cardiac myofibril | larval stage, with Scer\GAL4^4XHand

Ubc6^RNAi.UAS.cUa

dorsal vessel wall cell | adult stage, with Scer\GAL4^4XHand

Ubc6^RNAi.UAS.cUa

embryonic/larval heart | larval stage, with Scer\GAL4^4XHand

Ubc6^RNAi.UAS.cUa

embryonic/larval neuromuscular junction | third instar larval stage

extracellular matrix | adult stage, with Scer\GAL4^4XHand

Ubc6^RNAi.UAS.cUa

extracellular matrix | larval stage, with Scer\GAL4^4XHand

Ubc6^RNAi.UAS.cUa

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

Ubc6^GD13294

Johnston organ | P-stage | somatic clone, with Scer\GAL4^Act.PU

Ubc6^HMS02466

mitochondrion | third instar larval stage

myofibril, with Scer\GAL4^Mef2.PR

Ubc6^GD13294

sarcomere, with Scer\GAL4^Mef2.PR

Ubc6^GD13294

scolopidium | P-stage | somatic clone, with Scer\GAL4^Act.PU

Ubc6^HMS02466

wing, with Scer\GAL4^en.PU

Ubc6^RNAi.UAS

Z disc, with Scer\GAL4^Mef2.PR

Ubc6^GD13294

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (40)

Hsap\UBE2B

13 of 14

Yes

Hsap\UBE2A

12 of 14

Yes

Hsap\UBE2U

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (41)

Rnor\Ube2b

13 of 14

Yes

Rnor\Ube2a

12 of 14

Yes

Rnor\Ube2u

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (43)

Mmus\Ube2a

12 of 14

Yes

Mmus\Ube2b

12 of 14

Yes

Mmus\Ube2u

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (21)

Xtro\ube2a

10 of 13

Yes

Xtro\ube2b

10 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ube2w

1 of 13

Xtro\ube2z

1 of 13

Danio rerio (Zebrafish) (44)

Drer\ube2al

13 of 14

Yes

Drer\ube2b

13 of 14

Yes

Drer\ube2a

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch1073-205c8.3

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (23)

Cele\ubc-1

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (26)

Agam\AgaP_AGAP003857

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (48)

Atha\UBC3

13 of 13

Yes

Atha\UBC1

12 of 13

Yes

Atha\UBC2

12 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

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1 of 13

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1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (13)

Scer\RAD6

11 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (14)

Spom\rhp6

11 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ubc6. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (32)

CG7656

3 of 13

CG16894

3 of 13

CG40045

3 of 13

lwr

3 of 13

peo

3 of 13

Ubc7

3 of 13

Ubc87F

3 of 13

ben

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

vih

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

eff

1 of 13

Kua

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 5 )

Allele

Disease

Evidence

References

Ubc6^HMS02466

model of congenital heart disease

CEA

(Zhu et al., 2017)

Ubc6^EY04634

model of syndromic intellectual disability

CEA

(Haddad et al., 2013)

Ubc6^GL00405

model of congenital heart disease

CEA

(Zhu et al., 2017)

Ubc6^GD13294

model of metabolic dysfunction-associated steatotic liver disease

CEA

(Zhao et al., 2023, Zhang et al., 2022)

Ubc6^Δ1

model of syndromic intellectual disability

CEA

(Haddad et al., 2013)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

UBE2A; ubiquitin conjugating enzyme E2 A

model of syndromic X-linked intellectual disability Nascimento type

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Ubc6^HMS02466

exacerbates cancer

modeled by Rbf^HMS03004

(Parkhitko et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

UBE2B; ubiquitin conjugating enzyme E2 B

13 of 14

179095

UBE2A; ubiquitin conjugating enzyme E2 A

12 of 14

312180

INTELLECTUAL DEVELOPMENTAL DISORDER, X-LINKED, SYNDROMIC, NASCIMENTO TYPE; MRXSN

syndromic X-linked intellectual disability Nascimento type

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ubc6 - asf1

anti tag coimmunoprecipitation, western blot, pull down

(Wang et al., 2015)

Ubc6 - mod(mdg4)

two hybrid

(Hu et al., 2017.6.13)

Ubc6 - p53

pull down, western blot, coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation

(Chen et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Otud6

suppressible

Ubc6

(Villa et al., 2024)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0004436

MIST (protein-protein) - An integrated Molecular Interaction Database

40610

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

protein ubiquitination

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ubiquitin mediated proteolysis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P25153

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47.1

Cytogenetic map

82E3-82E4

Sequence location

3R:4,948,755..4,952,682 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

82E3-82E4

Limits computationally determined from genome sequence between P{PZ}l(3)02733⁰²⁷³³ and P{EP}EP974

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-47.1

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

42631

y¹ v¹; P{TRiP.HMS02466}attP40

80352

y¹ v¹; P{TKO.GS03322}attP40/CyO

22751

y¹ w^67c23; Mi{ET1}Ubc6^MB00268

35476

y¹ sc^* v¹ sev²¹; P{TRiP.GL00405}attP2

79495

y¹ sc^* v¹ sev²¹; P{TOE.GS01874}attP40

77416

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06548}attP2

35975

y¹ w^*; Mi{MIC}Ubc6^MI02360/TM3, Sb¹ Ser¹

81558

w^*; l(2)*^*/CyO; P{ST.lexA::HG}Ubc6^SE101/TM6B, Tb¹

Kyoto

123489

w¹¹¹⁸; P{RS3}Ubc6^CB-5152-3

NIG-Fly

HMS02466

y¹ v¹; P{TRiP.HMS02466}attP40/CyO

VDRC

v23229

w¹¹¹⁸; P{GD13294}v23229

v46927

w¹¹¹⁸; P{GD17232}v46927/TM3

More stocks available...

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (85)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE56673

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004436

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004436

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Mazina et al., 2018)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

UbcD6 negatively regulates p53 turnover.

(Chen et al., 2011)

UbcD6 is involved in centrosome separation.

(Dobbelaere et al., 2008)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in the formation of monastral bipolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cells does not affect the number of prophase centrosomes, but monastral bipolar spindles are formed during mitosis, suggesting that the centrosomes are fused.

(Goshima et al., 2007)

Area matching Drosophila RAD6 gene, Acc. No. M63792.

(Davies, 2001.3.15)

Isolated from a genomic library using a Scer\rad6 DNA fragment and a S.pombe rhp6 DNA fragment as probes.

(Koken et al., 1991)

UbcD6 has been cloned and sequenced.

(Koken et al., 1991)

Relationship to Other Genes

Source for database merge of

Source for merge of: UbcD6 BcDNA:RE56673

(FlyBase, 1996-)

Additional comments

Source for merge of UbcD6 BcDNA:RE56673 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

UbcD6 increases the UV resistance of S.cerevisiae Scer\rad6 mutant cells, although not to wild-type levels. The γ ray sensitivity of S.cerevisiae Scer\rad6 mutant cells is also complemented by UbcD6, to almost wild-type levels. The sporulation defect of S.cerevisiae Scer\rad6 mutant cells is not rescued by UbcD6.

(Koken et al., 1991)

Nomenclature History

Source for database identify of

Source for identity of: UbcD6 CG2013

(Guan, 1999.11)

Source for identity of: Ubc6 UbcD6

(FlyBase, 2009-)

Nomenclature comments

Renamed from 'UbcD6' to 'Ubc6' as the 'D' indicates 'Drosophila' (which FlyBase nomenclature rules disallow).

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

BcDNA:RE56673

CG2013

(Ibaraki et al., 2021, Park et al., 2016, Hosono et al., 2015, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Ardehali et al., 2009, Perkins et al., 2009, Dobbelaere et al., 2008, Goshima et al., 2007)

Dhr6

(Speese et al., 2003, Oh et al., 1994, Prakash et al., 1993, Koken et al., 1991)

RAD6

(Higa et al., 2003)

Rad6

(Galletta et al., 2023, Cai et al., 2022, Zanet et al., 2015, Chen et al., 2011, Speese et al., 2003)

Rad6a

(Haddad et al., 2013)

UBC6

(Ashton-Beaucage et al., 2016)

Ubc6

(Sutton et al., 2024, Villa et al., 2024, Li et al., 2023, Nieken et al., 2023, Ibaraki et al., 2021, Mahesh et al., 2020, Kockel et al., 2019, Hu et al., 2017.6.13, Transgenic RNAi Project members, 2017-, Zanet et al., 2016, Kavi et al., 2015)

Ubcd6

(Zhu et al., 2017)

dRAD6

(Haddad et al., 2013)

dRad6

(Wang et al., 2015, Haddad et al., 2013)

Name Synonyms

Ubiquitin Conjugating Enzyme

(Kaplow et al., 2007)

Ubiquitin conjugating enzyme

Ubiquitin conjugating enzyme 6

Secondary FlyBase IDs

FBgn0064873

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 53 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40610

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P25153

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P25153

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4K5Z5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P25153

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40610/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004436

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004436

FlyMine - An integrated database for Drosophila genomics

FBgn0004436

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2013

MARRVEL_MODEL - MARRVEL (model organism gene)

40610

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004436

DroID - A comprehensive database of gene and protein interactions.

FBgn0004436

DRSC - Results frm RNAi screens

FBgn0004436

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ubc6_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004436

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004436

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

protein ubiquitination

Flygut - An atlas of the Drosophila adult midgut

FBgn0004436

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0004436

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011188

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ubiquitin mediated proteolysis

MIST (protein-protein) - An integrated Molecular Interaction Database

40610

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P25153

References (103)

The following external sites may use different assemblies or annotations than FlyBase.

-1.78

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