Ubc13, Bend, UbcD3
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.45
Gene model reviewed during 5.50
2.0, 1.2 (northern blot)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ben using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\ben in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: ben CG18319
Source for merge of: ben anon-WO03040301.242
ben does not seem to be required for nascent synapse formation but rather plays a role in synaptic growth and maturation.
ben functions as a novel developmental switch that permits the transition from axonal growth and incipient synapse formation to synaptic growth and maturation in the central nervous system.
dsRNA made from templates generated with primers directed against this gene.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
ben mRNA is terminated at the poly(A) signal provided by the Ste intergenic spacer 3' untranslated region which is present upstream of the neighbouring Ste12DOR coding region. Overall orientation not stated: ben+ Ste12DOR+
Mutants have pleiotropic phenotypes affecting thoracic muscle patterning, pupal mortality and post-eclosion mortality.
Mutations in ben cause a disruption in the recognition event leading to synapse formation between the giant fibre (GF) and the tergotrochanteral jump muscle motorneuron (TTMmn) giving rise to the GF phenotype. Also mutants exhibit morphological abnormalities within the visual system. Results suggest a role for ben for ubiquitin-mediated protein modification in nervous system development, including the regulation of synaptic connectivity.
Adults have an aberrant startle response; they do not jump when presented with a lights-off stimulus. The cervical giant fiber, a brain neuron, has abnormal morphology. The normal giant fiber terminates in the thorax at a synapse onto the motoneuron innervating the tergo-trochanteral (TT) muscle (= jump muscle). In ben, this synapse is abnormal or absent. The normal lateral bend of the giant fiber toward the TT motoneuron in the mesothoracic neuromere is absent; the axon usually terminates at the midline with fine branches extending from its tip. Latency of response of the TT muscle to stimulation of the giant fiber is abnormally long, and muscles cannot follow stimulation at rates above 5 Hz. Rhabdomeres of the photoreceptor cells in the ommatidia heart shaped in cross-section with indentation centrally oriented rather than round as is normal. Furthermore, the axons from photoreceptor cells R7 and R8 fail to make the right-angle turn into the optic medulla after traversing the lamina. ben flies choose visible over ultraviolet wave lengths whereas wild-type flies make the opposite choice.
Less than 25% ben flies show abnormalities in the dorsal attachment of the tergotrochantral muscle.
Behavioural data suggests antennal and maxillary basiconic sensilla may be important receptors for short chain alcohols and organic acids but less crucial for acetates, aldehydes and ketones.
Mutations have a defect in the pathway between the giant fibre and jump muscle, the giant fiber fails to make a bend which would normally bring it into proximity with the jump muscle motoneuron.
Mutations interrupt one neural connection in the giant fibre pathway that mediates the jump/flight escape response.
Mutations disrupt the synaptic transmission between the giant fibre (GF) system and the tergotrochanteral muscle (TTM, jumping muscle) motor neuron.
Named 'UbcD3' for 'ubiquitin-conjugating enzyme from Drosophila 3 (the numbering reflects the order of identification).