FlyBase Allele Report: Dmel\ben[GD1387]

General Information

Symbol

Dmel\ben^GD1387

Species

D. melanogaster

Name

FlyBase ID

FBal0209139

Feature type

allele

Associated gene

Dmel\ben

Associated Insertion(s)

Carried in Construct

P{GD1387}

Also Known As

UAS-ben-IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1387}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1387

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS, l(2)gl⁴

(Ma et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Expression of ben^GD1387 ameliorates tumor-like growth and invasion when Ras85D^V12.Scer\UAS is expressed in l(2)gl⁴ mutant cells.

(Ma et al., 2014)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

short lived, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

some die during pharate adult stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Down-regulation of ben, through expression of ben^GD1387 under the control of Scer\GAL4^Mhc.PW results in no change in overall muscular mitochondria morphology in the thorax.

Down-regulation of ben, through expression of ben^GD1387 under the control of Scer\GAL4^Mhc.PW does not affect mitochondrial ATP production.

(Shiba-Fukushima et al., 2014)

Adults expressing ben^GD1387 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in lethality by 7 days after eclosion.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in mild thorax closure defects.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1387

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ben^GD1387, Uev1A^GD6650, Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

NOT Enhancer of

Statement

Reference

Scer\GAL4^da.PU/ben^GD1387, Scer\GAL80^ts.αTub84B is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

ben^GD1387/Scer\GAL4^GMR.PF is a non-enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

Suppressor of

Statement

Reference

ben^GD1387/Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2014)

ben^GD1387/Scer\GAL4^pnr.PU is a suppressor | partially of visible phenotype of Scer\GAL4^pnr.PU, egr^UAS.cIa

(Ma et al., 2014)

ben^GD1387, Scer\GAL4^FRT.Act5C is a suppressor of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2014)

ben^GD1387/Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/ben^GD1387, Scer\GAL80^ts.αTub84B is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Other

Statement

Reference

Scer\GAL4^GMR.PU, ben^+t6, ben^GD1387, egr^UAS.cIa has visible phenotype

(Ma et al., 2014)

Scer\GAL4^GMR.PU, ben^EP1651, ben^GD1387, egr^UAS.cIa has visible phenotype

(Ma et al., 2014)

Scer\GAL4^GMR.PU, ben^GD1387, ben^d09936, egr^UAS.cIa has visible phenotype

(Ma et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

ben^GD1387, Uev1A^GD6650, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

NOT Enhancer of

Statement

Reference

ben^GD1387/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

Suppressor of

Statement

Reference

ben^GD1387/Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Ma et al., 2014)

ben^GD1387/Scer\GAL4^pnr.PU is a suppressor | partially of scutellum phenotype of Scer\GAL4^pnr.PU, egr^UAS.cIa

(Ma et al., 2014)

ben^GD1387/Scer\GAL4^ptc.PU is a suppressor of anterior-posterior compartment boundary of the wing disc phenotype of Scer\GAL4^ptc.PU, scrib^GD11666

(Ma et al., 2014)

ben^GD1387/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

Other

Statement

Reference

Scer\GAL4^GMR.PU, ben^+t6, ben^GD1387, egr^UAS.cIa has eye phenotype

(Ma et al., 2014)

Scer\GAL4^GMR.PU, ben^EP1651, ben^GD1387, egr^UAS.cIa has eye phenotype

(Ma et al., 2014)

Scer\GAL4^GMR.PU, ben^GD1387, ben^d09936, egr^UAS.cIa has eye phenotype

(Ma et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ben^GD1387 strongly suppresses the small eye phenotype seen in flies expressing egr^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PU. This suppression is partially reverted upon expression of either ben^+t6, ben^EP1651 or ben^d09936.

Expression of ben^GD1387 strongly suppresses the scutellum phenotype seen when egr^Scer\UAS.cIa is expressed under the control of Scer\GAL4^pnr.PU.

Expression of ben^GD1387 significantly suppresses the growth and invasion of the tumors seen when Ras85D^V12.Scer\UAS is expressed in l(2)gl⁴ mutant cells in eye-antennal discs using the ey-FLP/MARCM system.

Expression of ben^GD1387 suppresses the cell migration seen when scrib^GD11666 is expressed along the anterior/posterior (A/P) compartment boundary of wing imaginal discs under the control of Scer\GAL4^ptc.PU.

(Ma et al., 2014)

Loss of ben, through expression of ben^GD1387, significantly suppresses the Scer\GAL4^GMR.PF->nopo^Scer\UAS.cUa small rough eye phenotype.

(Ma et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v9413

w¹¹¹⁸; P{GD1387}v9413

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1387

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ben^GD1387

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close