FlyBase Gene Report: Dmel\nopo

General Information

Symbol

Dmel\nopo

Species

D. melanogaster

Name

no poles

Annotation Symbol

CG5140

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034314

Gene Model Status

Current

Stock Availability

6 publicly available

Enzyme Name (EC)

Gene Summary

no poles (nopo) encodes a RING domain-containing E3 ubiquitin ligase that is essential for early embryogenesis. It positively regulates caspase-dependent cell death. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

55B12-55B12

Sequence location

2R:18,180,496..18,182,519 [+]

Recombination map

2-84

RefSeq locus

NT_033778 REGION:18180496..18182519

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables DNA polymerase binding

inferred from physical interaction with FLYBASE:PolH; FB:FBgn0037141

(Wallace et al., 2014)

enables ubiquitin conjugating enzyme binding

inferred from physical interaction with FLYBASE:ben; FB:FBgn0000173

(Merkle et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ubiquitin protein ligase activity

inferred from sequence or structural similarity with UniProtKB:Q8VIG6

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN002505736

(GO Reference Genome Project, 2011-)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR011016

(InterPro Project Members, 2004-)

inferred from sequence model

(Ying et al., 2011)

Biological Process (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in lysosomal protein catabolic process

inferred from direct assay

(Lee et al., 2018)

involved_in positive regulation of apoptotic process

inferred from mutant phenotype

(Ma et al., 2012)

involved_in positive regulation of mitotic cell cycle, embryonic

inferred from mutant phenotype

(Merkle et al., 2009)

involved_in positive regulation of tumor necrosis factor-mediated signaling pathway

inferred from genetic interaction with FLYBASE:egr; FB:FBgn0033483

(Ma et al., 2012)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in protein ubiquitination

inferred from sequence or structural similarity with MGI:MGI:1096377

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN002505736

(GO Reference Genome Project, 2011-)

involved_in replication fork processing

inferred from biological aspect of ancestor with PANTHER:PTN002505736

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from direct assay

(Merkle et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002505736

(GO Reference Genome Project, 2011-)

is_active_in site of DNA damage

inferred from biological aspect of ancestor with PANTHER:PTN002505736

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED RING DOMAIN UBIQUITIN LIGASES

Pathway (FlyBase)

POSITIVE REGULATORS OF TNFalpha-EIGER SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ubiquitin protein ligase activity

Summaries

Gene Snapshot

no poles (nopo) encodes a RING domain-containing E3 ubiquitin ligase that is essential for early embryogenesis. It positively regulates caspase-dependent cell death. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

UNCLASSIFIED RING DOMAIN UBIQUITIN LIGASES -: This group comprises RING domain ubiquitin ligases that do not classify under other groups in FlyBase.

Pathway (FlyBase)

POSITIVE REGULATORS OF TNFalpha-EIGER SIGNALING PATHWAY -: Positive regulators of Tumor Necrosis Factor α (TNFα) signaling pathway up-regulate the activation of the pathway triggered by Eiger (egr).

Summary (Interactive Fly)

E3 ubiquitin ligase required for the preservation of genomic integrity during early embryogenesis

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\nopo for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7K2X1)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

nopo-RA

FBtr0086750

NM_137461

1553

435

nopo-RB

FBtr0332852

NM_001274142

1671

434

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

nopo-PA

FBpp0085929

48.5

435

9.75

FBpp0305075

48.3

434

9.67

A0A0B4KFV0

NP_001261071

AGB93603

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nopo using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.25

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

is_active_in nucleus

inferred from direct assay

(Merkle et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\nopo in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\nopo

View exonic expression by tissue for Dmel\nopo

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034314

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37083/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0034314

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034314

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0034314

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5140

Flygut - An atlas of the Drosophila adult midgut

FBgn0034314

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 22 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of nopo

Transgenic constructs containing regulatory region of nopo

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

Df(2R)BSC337

(Ma et al., 2012)

Df(2R)Exel7152

(Ryder, 2004.4.26)

Df(2R)Exel7153

(Ma et al., 2012, Merkle et al., 2009, Ryder, 2004.4.26)

Df(2R)PC4

(Merkle et al., 2009)

Df(2R)Pcl7B

(Merkle et al., 2009)

Df(2R)Pcl11B

(Merkle et al., 2009)

Df(2R)Pcl^XM82

(Merkle et al., 2009)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | maternal effect | recessive

nopo^Exc142

lethal - all die during embryonic stage | non-rescuable maternal effect (with Df(2R)Exel7153)

lethal - all die during embryonic stage | non-rescuable maternal effect | recessive

lethal - all die during embryonic stage | non-rescuable maternal effect | recessive | heat sensitive

nopo^5-SZ-3004

partially lethal | maternal effect

nopo^5-SZ-3004

viable

nopo^Exc142

viable, with Scer\GAL4^Mef2.PR

nopo^GD11550

viable, with Scer\GAL4^pnr-MD237

nopo^GD11550

viable, with Scer\GAL4^tin.CΔ4

nopo^GD11550

Sterility

Allele

female sterile (with Df(2R)Exel7153)

female sterile (with Df(2R)PC4)

nopo^Z1447

female sterile (with Df(2R)Pcl7B)

nopo^Z1447

female sterile (with Df(2R)Pcl11B)

nopo^Z1447

female sterile (with Df(2R)Pcl^XM82)

nopo^Z1447

female sterile | recessive

Other Phenotypes

Allele

abnormal developmental rate | maternal effect

nopo^5-SZ-3004

decreased size | adult stage, with Scer\GAL4^unspecified

nopo^KK109198

increased cell death, with Scer\GAL4^GMR.PF

nopo^UAS.cUa

increased cell death, with Scer\GAL4^sd-SG29.1

nopo^UAS.cUa

majority die during embryonic stage | maternal effect

nopo^5-SZ-3004

some die during embryonic stage | non-rescuable maternal effect (with Df(2R)Exel7153)

some die during embryonic stage | recessive

some die during embryonic stage | recessive | heat sensitive

nopo^5-SZ-3004

visible | adult stage, with Scer\GAL4^unspecified

nopo^KK109198

Phenotype manifest in

Allele

centrosome | non-rescuable maternal effect

nopo^Z1447

eye, with Scer\GAL4^GMR.PF

nopo^UAS.cUa

eye, with Scer\GAL4^unspecified

nopo^KK109198

mitotic spindle | maternal effect

spindle | non-rescuable maternal effect

nopo^Z1447

wing margin, with Scer\GAL4^sd-SG29.1

nopo^UAS.cUa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (44)

Hsap\TRAIP

11 of 14

Yes

Hsap\BRCA1

1 of 14

Hsap\COP1

1 of 14

Yes

Hsap\DZIP3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\TRIM8

1 of 14

Yes

Hsap\TRIM11

1 of 14

Yes

Hsap\TRIM22

1 of 14

Yes

Hsap\TRIM31

1 of 14

Yes

Hsap\TRIM35

1 of 14

Yes

Hsap\TRIM50

1 of 14

Yes

Hsap\TRIM60

1 of 14

Yes

Hsap\TRIM62

1 of 14

Yes

Hsap\TRIM68

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (26)

Rnor\Traip

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Trim31

1 of 14

Yes

Rnor\Trim50

1 of 14

Yes

Rnor\Trim62

1 of 14

Yes

Rnor\Zswim2

1 of 14

Yes

Mus musculus (laboratory mouse) (30)

Mmus\Traip

10 of 14

Yes

Mmus\Bfar

1 of 14

Yes

Mmus\Dzip3

1 of 14

Yes

Mmus\Golga3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Trim11

1 of 14

Yes

Mmus\Trim62

1 of 14

Yes

Mmus\Zswim2

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (26)

Xtro\traip

9 of 13

Yes

Xtro\brca1

1 of 13

Yes

Xtro\cdpf1

1 of 13

Xtro\chfr

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (32)

Drer\traip

11 of 14

Yes

Drer\bmi1b

1 of 14

Drer\cop1

1 of 14

Yes

Drer\ftr41

1 of 14

Drer\golm2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\trim35-33

1 of 14

Yes

Drer\trim35-40

1 of 14

Yes

Drer\trim105

1 of 14

Yes

Drer\trim108

1 of 14

Yes

Drer\ttc3

1 of 14

Yes

Drer\zmp:0000000662

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\trul-1

10 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\trf-1

1 of 14

Cele\Y52E8A.2

1 of 14

Yes

Cele\Y54E10BR.3

1 of 14

Cele\ZC13.1

1 of 14

Anopheles gambiae (African malaria mosquito) (21)

Agam\AgaP_AGAP006611

12 of 12

Yes

Agam\AgaP_AGAP007004

5 of 12

Yes

Agam\AgaP_AGAP006367

4 of 12

Yes

Agam\AgaP_AGAP013371

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (95)

Atha\AT4G01270

5 of 13

Yes

Atha\AIRP1

1 of 13

Atha\AT1G04360

1 of 13

Atha\AT1G08050

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT2G18670

1 of 13

Atha\AT2G27940

1 of 13

Atha\AT2G34000

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT3G51325

1 of 13

Atha\AT3G54780

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\RHA1A

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\ASR1

1 of 13

Yes

Scer\IRC20

1 of 13

Yes

Scer\SLX8

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\hrd1

1 of 12

Yes

Spom\SPAC144.05

1 of 12

Yes

Spom\SPBC2A9.04c

1 of 12

Yes

Spom\SPCC548.05c

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:nopo. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (31)

4 of 13

4 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

gol

1 of 13

gzl

1 of 13

mus302

1 of 13

Rnf11

1 of 13

Sce

1 of 13

sinah

1 of 13

sip3

1 of 13

Traf6

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Seckel syndrome 9

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 7 )

Allele

Disease

Evidence

References

nopo^Exc142

model of Seckel syndrome 9

CEA with nopo^Δ

(O'Neill and Rusan, 2022)

nopo^Δ

model of Seckel syndrome 9

CEA with nopo^Exc142

(O'Neill and Rusan, 2022)

nopo^{Δ173-268.UASp.EGFP}

model of Seckel syndrome 9

CEA

(O'Neill and Rusan, 2022)

nopo^{269-435.UASp.EGFP}

model of Seckel syndrome 9

CEA

(O'Neill and Rusan, 2022)

nopo^{173-435.UASp.EGFP}

model of Seckel syndrome 9

CEA

(O'Neill and Rusan, 2022)

nopo^{1-172.UASp.EGFP}

model of Seckel syndrome 9

CEA

(O'Neill and Rusan, 2022)

nopo^{I8D.A10E.UASp.EGFP}

model of Seckel syndrome 9

CEA

(O'Neill and Rusan, 2022)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

TRAIP; TRAF interacting protein

model of Seckel syndrome 9

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

nopo^{ΔNLS.UASp.EGFP}

ameliorates Seckel syndrome 9

modeled by nopo^Δ, nopo^Exc142

(O'Neill and Rusan, 2022)

nopo^{UAS.Tag:Mito(Tom20),TagRFP}

ameliorates Seckel syndrome 9

modeled by nopo^Δ, nopo^Exc142

(O'Neill and Rusan, 2022)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TRAIP; TRAF interacting protein

11 of 14

TRAF-INTERACTING PROTEIN; TRAIP

SECKEL SYNDROME 9; SCKL9

Seckel syndrome 9

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

nopo

Hsap\TRAIP

(Wallace et al., 2014)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

nopo

two hybrid

(Merkle et al., 2009)

nopo - PolH

two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Wallace et al., 2014)

nopo - PolI

two hybrid

(Wallace et al., 2014)

(Lee et al., 2018)

(Merkle et al., 2009)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Ma et al., 2012)

enhanceable

(Ma et al., 2012)

suppressible

(Ma et al., 2012)

suppressible

(Merkle et al., 2009)

nopo

suppressible

PolH

(Wallace et al., 2014)

suppressible

(Ma et al., 2012)

enhanceable

(Ma et al., 2012)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Lee et al., 2018)

enhanceable

(Ketosugbo et al., 2017)

cindr

suppressible

nopo

(Ketosugbo et al., 2017)

egr

suppressible

nopo

(Ma et al., 2012)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62764

DroID - A comprehensive database of gene and protein interactions.

FBgn0034314

MIST (genetic) - An integrated Molecular Interaction Database

37083

MIST (protein-protein) - An integrated Molecular Interaction Database

37083

Pathways

Signaling Pathways (FlyBase)

POSITIVE REGULATORS OF TNFalpha-EIGER SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Antigen processing: Ubiquitination & Proteasome degradation

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-84

Cytogenetic map

55B12-55B12

Sequence location

2R:18,180,496..18,182,519 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55B12-55B12

Limits computationally determined from genome sequence between P{PZ}Hsf⁰³⁰⁹¹&P{EP}Dgp-1^EP731 and P{PZ}sbb⁰⁴⁵²⁵&P{EP}EP1081^EP1081

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-84

(FlyBase, 2016)

2-88.7

(Comeron et al., 2012)

Left of (cM)

P{EPgy2}CG5721^EY03388

(Merkle et al., 2009)

Right of (cM)

P{GT1}Gtpbp1^BG00396

(Merkle et al., 2009)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

57335

w¹¹¹⁸; nopo^Exc142/CyO

27191

y¹ w^*; P{EP}nopo^G5845

57334

cn¹ nopo^Z1447 bw¹/CyO

VDRC

v104477

P{KK109198}VIE-260B

v22013

w¹¹¹⁸; P{GD11550}v22013/TM3

v342751

P{hsFLP}1, y1 w1118; P{HD_CFD01669}attP40/CyO-GFP

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (159)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH03577

Other clones

DPiM_GH03577

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0034314

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0034314

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Merkle et al., 2009)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Embryos from nopo females undergo mitotic arrest with a high frequency of acentrosomal spindles, misaligned chromosomes, and tripolar spindles.

(Merkle et al., 2007)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: nopo CG5140

(Merkle et al., 2007)

Nomenclature comments

Etymology

The gene is named "no poles" based on the phenotype of acentrosomal mitotic spindles in mutant-derived embryos.

(Merkle et al., 2009)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG5140

(Ying et al., 2011, Keleman et al., 2009.8.5, Merkle et al., 2009, Merkle and Lee, 2008)

NOPO

(Colombani and Andersen, 2023, Loudhaief et al., 2023, Lee et al., 2018, Ma et al., 2012)

Nopo

(Von Stetina et al., 2008)

TRIP

(Merkle and Lee, 2008)

Traip

(O'Neill and Rusan, 2022)

nopo

(O'Neill and Rusan, 2022, Bilder et al., 2021, Ketosugbo et al., 2017, Appel et al., 2015, Kavi et al., 2015, Wallace et al., 2014, Du et al., 2011, Merkle et al., 2009, Merkle and Lee, 2008, Garcia et al., 2007, Merkle et al., 2007)

Name Synonyms

CG5140

no poles

(Ma et al., 2012, Merkle et al., 2009, Merkle and Lee, 2008, Garcia et al., 2007, Merkle et al., 2007)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37083

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7K2X1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7K2X1

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0034314

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37083/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0034314

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0034314

FlyMine - An integrated database for Drosophila genomics

FBgn0034314

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5140

MARRVEL_MODEL - MARRVEL (model organism gene)

37083

Linkouts

BioGRID - A database of protein and genetic interactions.

62764

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0034314

DroID - A comprehensive database of gene and protein interactions.

FBgn0034314

DRSC - Results frm RNAi screens

FBgn0034314

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034314

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0034314

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5140

Flygut - An atlas of the Drosophila adult midgut

FBgn0034314

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0034314

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015952

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

37083

MIST (protein-protein) - An integrated Molecular Interaction Database

37083

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Antigen processing: Ubiquitination & Proteasome degradation

References (44)

The following external sites may use different assemblies or annotations than FlyBase.

1.25

Report Sections