NOPO
fused to GAL4 DNA-binding domain
dPolη
fused to GAL4 activation domain
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
nopo interacted with DNApol-η and DNApol-ι, but not with Rev1 (inconclusive), in yeast two hybrid assays.
Myc tag
HA tag
nopo coimmunoprecipitates DNApol-η, but not DNApol-ι, in this assay.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
