FlyBase Allele Report: Dmel\nopo[Exc142]

General Information

Symbol

Dmel\nopo^Exc142

Species

D. melanogaster

Name

FlyBase ID

FBal0239901

Feature type

allele

Associated gene

Dmel\nopo

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Merkle et al., 2009)

Progenitor genotype

P{EP}nopo^G5845

(Merkle et al., 2009)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a 771bp deletion that lacks part of the 5' UTR and exons encoding amino acid residues 1-181.

(Merkle et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:18,180,610..18,181,380 [+]

Comment:

Imprecise excision of P{EP}nopoG5845, resulting in a 771bp deletion that lacks part of the 5' UTR and exons encoding amino acid residues 1-181.

(Merkle et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Seckel syndrome 9

CEA with nopo^Δ

(O'Neill and Rusan, 2022)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mitotic spindle | maternal effect

(Wallace et al., 2014)

Detailed Description

Statement

Reference

Embryos derived from nopo^Exc142 females fail to hatch. 94% of the embryos have abnormal spindle morphology.

(Wallace et al., 2014)

15% of embryos derived from homozygous females mated to wild-type males develop to cycle 10 or beyond, but none of these embryos develop to the initiation of gastrulation or beyond.

7% of embryos derived from nopo^Exc142/Df(2R)Exel7153 females mated to wild-type males develop to cycle 10 or beyond, but none of these embryos develop to the initiation of gastrulation or beyond.

(Merkle et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

nopo^Exc142 has lethal - all die during embryonic stage | recessive | maternal effect phenotype, suppressible | partially by Hsap\TRAIP^nopo.PW

(Wallace et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

nopo^Exc142 has mitotic spindle | maternal effect phenotype, suppressible | partially by Hsap\TRAIP^nopo.PW

(Wallace et al., 2014)

Suppressor of

Statement

Reference

nopo^Exc142/nopo^Exc142 is a suppressor of endosome | adult stage phenotype of Cad99C^UAS.GFP, Scer\GAL4^NP0001

(Lee et al., 2018)

nopo^Exc142/nopo^Exc142 is a suppressor of adult enterocyte phenotype of Cad99C^UAS.GFP, Scer\GAL4^NP0001

(Lee et al., 2018)

Other

Statement

Reference

lok^p6, nopo^Exc142/nopo^Z1447 has interphase & organism | embryonic cycle 11 | maternal effect phenotype

(Merkle et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos derived from lok^p6 nopo^Z1447/lok^p6 nopo^Exc142 females have significantly shorter interphases during cycle 11 compared to wild-type or lok^p6 single mutant embryos.

(Merkle et al., 2009)

Xenogenetic Interactions

Statement

Reference

Hsap\TRAIP^nopo.PW rescues the hatch rate of embryos derived from nopo^Exc142 females from 0% to 83%, and the frequency of embryos with abnormal spindle morphology is reduced from 94% to 14%.

(Wallace et al., 2014)

Complementation and Rescue Data

Rescued by

nopo^Exc142 is rescued by nopo^+t3.8

(Merkle et al., 2009)

Partially rescued by

nopo^Exc142 is partially rescued by nopo^cDNA

(Wallace et al., 2014)

Comments

nopo^cDNA rescues the hatch rate of embryos derived from nopo^Exc142 females from 0% to 76%, and the frequency of embryos with abnormal spindle morphology is reduced from 94% to 22%..

(Wallace et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

57335

w¹¹¹⁸; nopo^Exc142/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

NOPO^Exc142

(Lee et al., 2018)

Traip^Exc142

(O'Neill and Rusan, 2022)

nopo^Exc142

(Wallace et al., 2014, Ma et al., 2012, Merkle et al., 2009)

nopo^excl42

(Ketosugbo et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close