FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
asf1, Ubc6
FlyBase ID
FBig0000110863
Interaction Network
Interactions Browser links
asf1 network
Ubc6 network
Reported Interactions
FBrf0233077-1.PD.WB
Reference
Wang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubc6 

Ubc6

bait

GST tag

asf1 

asf1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derivation unspecified.

(Wang et al., 2015)
FBrf0233077-2.coIP.WB
Reference
Wang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ubc6 

Ubc6

bait

HA tag

asf1 

asf1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Wang et al., 2015)

Co-IP assay done in presence of proteasome inhibitor, MG132.

(Wang et al., 2015)
FBrf0233077-3.coIP.WB
Reference
Wang et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
asf1 

asf1

bait

GFP tag

Ubc6 

Ubc6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Wang et al., 2015)

Co-IP assay done in presence of proteasome inhibitor, MG132. Treatment of transfected cells with DNA-damaging reagent, MMS, increases asf1-Ubc6 interaction.

(Wang et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)