FlyBase Allele Report: Dmel\mnb[UAS.cHa]

General Information

Symbol

Dmel\mnb^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hong

FlyBase ID

FBal0290881

Feature type

allele

Associated gene

Dmel\mnb

Associated Insertion(s)

Carried in Construct

P{UAS-mnb.H}

Also Known As

UAS-mnb

Key Links

Transgenic product class

wild_type

(Hong et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hong et al., 2012)

Mutagen

in vitro construct

(Hong et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-mnb.H}

Cytology

Description

UAS regulatory sequences drive expression of the full-length coding sequence of mnb.

(Hong et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mnb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Down syndrome

CEA

(Lowe et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior | adult stage, with Scer\GAL4^sNPF-R.2.5

(Hong et al., 2012)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav-C155

(Kim et al., 2016)

partially lethal - majority die, with Scer\GAL4^elav-C155

(Kim et al., 2016)

visible, with Scer\GAL4^Bx-MS1096

(Kim et al., 2016)

Phenotype Manifest In

larval central nervous system | embryonic stage, with Scer\GAL4^elav-C155

(Kim et al., 2016)

larval peripheral nervous system | embryonic stage, with Scer\GAL4^elav-C155

(Kim et al., 2016)

wing vein L5, with Scer\GAL4^Bx-MS1096

(Kim et al., 2016)

Detailed Description

Statement

Reference

Over-expression of mnb^Scer\UAS.cHa driven in the wing by Scer\GAL4^Bx-MS1096 results in incomplete formation of lateral vein 5 (90% penetration); over-expression in the eye driven by Scer\GAL4^GMR.PFa does not significantly reduce eye size. Over-expression of mnb^Scer\UAS.cHa driven pan-neuronally by Scer\GAL4^elav-C155 results in severe disorganization of the central and peripheral nervous systems at late embryonic stage 18, and high adult lethality (70% lethal).

(Kim et al., 2016)

Flies expressing mnb^Scer\UAS.cHa under the control of Scer\GAL4^sNPF-R.2.5 show significantly increased food consumption compared to controls.

Expression of mnb^Scer\UAS.cHa under the control of Scer\GAL4^Ilp2.PR has no significant effect on food intake in adults.

Adults expressing mnb^Scer\UAS.cHa under the control of Scer\GAL4^sNPF-R.2.5 have an increased body weight relative to controls.

(Hong et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^sNPF-R.2.5, mnb^UAS.cHa has abnormal feeding behavior | adult stage phenotype, suppressible by Scer\GAL4^sNPF-R.2.5/Sirt1^GD11580

(Hong et al., 2012)

Scer\GAL4^sNPF-R.2.5, mnb^UAS.cHa has abnormal feeding behavior | adult stage phenotype, suppressible by Scer\GAL4^sNPF-R.2.5/foxo^KK108485

(Hong et al., 2012)

Enhancer of

Statement

Reference

mnb^UAS.cHa, Scer\GAL4^GMR.PFa is an enhancer of visible phenotype of Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PFa

(Kim et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

mnb^UAS.cHa, Scer\GAL4^GMR.PFa is an enhancer of eye phenotype of Hsap\MAPT^UAS.wt, Scer\GAL4^GMR.PFa

(Kim et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in food consumption compared to controls seen in adults expressing mnb^Scer\UAS.cHa under the control of Scer\GAL4^sNPF-R.2.5 is suppressed by co-expression of either Sir2^GD11580 or foxo^KK108485.

(Hong et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mnb^Scer\UAS.cHa

mnb^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hong

Secondary FlyBase IDs

References (5)

Report Sections

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