FlyBase Allele Report: Rnor\Otc[d.UAS]

General Information

Symbol

Rnor\Otc^d.UAS

Species

R. norvegicus

Name

FlyBase ID

FBal0291052

Feature type

allele

Associated gene

Rnor\Otc

Associated Insertion(s)

Carried in Construct

P{UAS-rOTC.d}

Transgenic product class

wild_type

(Pimenta de Castro et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Pimenta de Castro et al., 2012)

Mutagen

in vitro construct

(Pimenta de Castro et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-rOTC.d}

Cytology

Description

UAS regulatory sequences drive expression of a mutant form of Rnor\Otc which produces an unfolded form of the protein.

(Pimenta de Castro et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rnor\Otc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

short lived, with Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

visible, with Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

Phenotype Manifest In

indirect flight muscle cell, with Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

mitochondrial crista, with Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

mitochondrion, with Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

wing, with Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

Detailed Description

Statement

Reference

A held-up wing phenotype is seen in 10-15% of flies expressing Rnor\Otc^d.Scer\UAS under the control of Scer\GAL4^da.G32 beginning at 3 days after eclosion. The indirect flight muscles contain mitochondrial aggregates. Ultrastructurally, the muscle structure is highly disorganised, and vacuolisation and fragmentation of mitochondrial cristae is seen. The flies show an age-dependent impairment in climbing ability and are short lived compared to wild type.

(Pimenta de Castro et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rnor\Otc^d.UAS, Scer\GAL4^da.G32 has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by park^UAS.cGa, Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rnor\Otc^d.UAS, Scer\GAL4^da.G32 has mitochondrion phenotype, suppressible by park^UAS.cGa, Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

Rnor\Otc^d.UAS, Scer\GAL4^da.G32 has indirect flight muscle cell phenotype, suppressible by park^UAS.cGa, Scer\GAL4^da.G32

(Pimenta de Castro et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of park^Scer\UAS.cGa rescues the age-dependent defect in climbing ability seen in flies expressing Rnor\Otc^d.Scer\UAS under the control of Scer\GAL4^da.G32. The defects in indirect flight muscle structure and mitochondrial integrity are also suppressed.

Co-expression of park^Scer\UAS.cGa is no longer able to suppress the defects in climbing ability and indirect flight muscle structure seen in flies expressing Rnor\Otc^d.Scer\UAS under the control of Scer\GAL4^da.G32 when the flies are also mutant for Atg1^Δ3D.

(Pimenta de Castro et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

605987

w^*; P{UAS-rOTC.d}29/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rnor\Otc^d.Scer\UAS

Rnor\Otc^d.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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