FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\spirY232K.UASp.RB.GFP
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General Information
Symbol
Dmel\spirY232K.UASp.RB.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0291757
Feature type
allele
Associated gene
Dmel\spir
Associated Insertion(s)
Carried in Construct
P{UASp-spir.RB.Y232K.GFP}
Key Links
Transgenic product class
missense_variant
(Quinlan, 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Quinlan, 2013)
Mutagen
in vitro construct
(Quinlan, 2013)
Progenitor genotype
Carried in construct
P{UASp-spir.RB.Y232K.GFP}
Cytology
Description

UASp regulatory sequences drive expression of a Y232K mutant of the spir isoform RB fused with a C-terminal GFP.

(Quinlan, 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
spir
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      spir1/Df(2L)Exel6046 females expressing Scer\GAL4nos.UTR.T:Hsim\VP16>spirY232K.Scer\UAS.P\T.RB.T:Avic\GFP are sterile. The mutant oocytes have no detectable actin mesh during mid-oogenesis and consistently exhibit premature streaming. At later stages of oogenesis, no mesh is visible and fast streaming is observed at stage 11.

      (Quinlan, 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4VP16.nanos.UTR, spirY232K.UASp.RB.GFP, Df(2L)Exel6046 fails to rescue spir1

      (Quinlan, 2013)
      Comments

      spir1/Df(2L)Exel6046 females expressing Scer\GAL4nos.UTR.T:Hsim\VP16>spirY232K.Scer\UAS.P\T.RB.T:Avic\GFP are sterile. The mutant oocytes have no detectable actin mesh during mid-oogenesis and consistently exhibit premature streaming. Furthermore, streaming velocities are indistinguishable from spir1/Df(2L)Exel6046 mutants.

      (Quinlan, 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      spirY232K.Scer\UAS.P\T.RB.T:Avic\GFP
      spirY232K.UASp.RB.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)