FlyBase Allele Report: Dmel\elg1[HMC03187]

General Information

Symbol

Dmel\elg1^HMC03187

Species

D. melanogaster

Name

FlyBase ID

FBal0292364

Feature type

allele

Associated gene

Dmel\elg1

Associated Insertion(s)

Carried in Construct

P{TRiP.HMC03187}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMC03187}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMC03187

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle | adult stage, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Huang et al., 2016)

Phenotype Manifest In

egg chamber, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Huang et al., 2016)

nurse cell, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Huang et al., 2016)

Detailed Description

Statement

Reference

Expression of elg1^HMC03187 RNAi under the control of the triple Scer\GAL4^nos.PG,Scer\GAL4^{nos.UTR.T:Hsim\VP16}, Scer\GAL4^{otu.T:Hsim\VP16} driver results in nurse cell endoreplication defects and underreplicated phenotype. Unlike controls, ovaries from elg1^HMC03187 RNAi expressing females lack cells with ploidy levels of 128C-1024C, from the ploidy levels that surpass those found also in somatic cells they display a peak only at 64C and the stage 14 egg chambers lack dorsal appendages.

(Huang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG, elg1^HMC03187 has abnormal endomitotic cell cycle | adult stage phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/enok^HMS02165, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Huang et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG, elg1^HMC03187 has abnormal endomitotic cell cycle | adult stage phenotype, suppressible | partially by enok^HMS02634/Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Huang et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG, elg1^HMC03187 has nurse cell phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/enok^HMS02165, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Huang et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG, elg1^HMC03187 has nurse cell phenotype, suppressible | partially by enok^HMS02634/Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Huang et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG, elg1^HMC03187 has egg chamber phenotype, suppressible | partially by enok^HMS02634/Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Huang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The underreplicated nurse cell phenotype characteristic for flies expressing elg1^HMC03187 RNAi under the control of the triple Scer\GAL4^nos.PG, Scer\GAL4^{nos.UTR.T:Hsim\VP16}, Scer\GAL4^{otu.T:Hsim\VP16} driver is partially suppressed by co-expression of either enok^HMS02165 RNAi or enok^HMS02634 RNAi. The nurse cells are still underreplicated but the nuclei are relatively bigger and the cells display higher ploidy levels compared to females expressing elg1^HMC03187 alone. enok^HMS02634 RNAi also partially rescues the missing dorsal appendages phenotype observed in elg1^HMC03187 RNAi expressing egg chambers.