Wild-type flies generate abundant pulse and sine courtship song. In contrast, most males expressing Hsap\KCNJ210x.Scer\UAS.T:Avic\GFP in both hg1 motorneurons under the control of Scer\GAL4GMR21A01 display a complete absence of sine song. Mosaic males with Hsap\KCNJ210x.Scer\UAS.T:Avic\GFP expressed in only one hg1 motorneuron produce a quantity of sine song intermediate between the flies that do not express or bilaterally express Hsap\KCNJ210x.Scer\UAS.T:Avic\GFP. All three categories of males produce statistically indistinguishable quantities of pulse song, including inter-pulse interval, pulse carrier frequencies, pulse amplitudes, pulse shapes, and pulse train lengths. The duration of the silent periods between bouts of song is similar in males of the three classes. The intrabout pauses, however, are lengthened in males expressing Hsap\KCNJ210x.Scer\UAS.T:Avic\GFP bilaterally compared with non-expressers. Simultaneous video and audio recording reveals that courting males with bilaterally silenced hg1 motorneurons extend a wing during periods of silence that often precede or follow trains of pulse song.
Genetic mosaics males expressing Hsap\KCNJ210x.Scer\UAS.T:Avic\GFP in hg1 motorneurons court females at levels statistically indistinguishable from controls, but they mate at a significantly lower rate than do controls.
Genetic mosaic males expressing Hsap\KCNJ210x.Scer\UAS.T:Avic\GFP in the motorneuron that innervates the ps1 flight control muscle under the control of Scer\GAL4GMR73C03 display a reduction in their courtship song pulse carrier frequency and pulse amplitude relative to controls but sing pulse and sine song otherwise normally.
