FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Nintra.CD8.UAS.EGFP
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General Information
Symbol
Dmel\Nintra.CD8.UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0293745
Feature type
allele
Associated gene
Dmel\N
Associated Insertion(s)
Carried in Construct
P{UAS-Nintra.CD8.eGFP}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Sanders et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Nintra.CD8.eGFP}
Cytology
Description

UAS regulates expression of a membrane-tethered version of N 'intra', with the extracellular and transmembrane domain of Mmus\Cd8a, that cannot be cleaved as it lacks intermembrane cleavage sites. Tagged with EGFP.

(Sanders et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
N
Tagged with
EGFP
Tag:M(mCd8a)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Nintra.Scer\UAS.T:Avic\GFP-EGFP,T:Mmus\Cd8a in cells of the wing disc results in adults with N gain-of-function phenotypes: loss of PNS precursors and veins.

      (Sanders et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL4hh-Gal4-mediated expression of Nintra.Scer\UAS.T:Avic\GFP-EGFP,T:Mmus\Cd8a suppresses the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1d7 wing discs.

      (Schneider et al., 2013)

      The over-proliferation defect of clones of cells mutant for N55e11 and expressing armS10.Scer\UAS.T:Hsap\MYC via Scer\GAL4αTub84B.PL (generated using the MARCM system) are rescued by co-expression of Nintra.Scer\UAS.T:Avic\GFP-EGFP,T:Mmus\Cd8a.

      Clones of cells mutant for N55e11 and expressing armS10.Scer\UAS.T:Hsap\MYC via Scer\GAL4dpp.PU are smaller and more frequent when Nintra.Scer\UAS.T:Avic\GFP-EGFP,T:Mmus\Cd8a is coexpressed.

      (Sanders et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Nintra.CD8.UAS.EGFP
      Nintra.Scer\UAS.T:Avic\GFP-EGFP,T:Mmus\Cd8a
      Name Synonyms
      Secondary FlyBase IDs
        References (2)