FlyBase Allele Report: Dmel\α-Cat[Ubi.PS]

General Information

Symbol

Dmel\α-Cat^Ubi.PS

Species

D. melanogaster

Name

FlyBase ID

FBal0294016

Feature type

allele

Associated gene

Dmel\α-Cat

Associated Insertion(s)

Carried in Construct

P{Ubi-α-Cat.S}

Also Known As

Ubi-α-Cat

Key Links

Transgenic product class

cDNA

(Sarpal et al., 2012)

wild_type

(Sarpal et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sarpal et al., 2012)

wild_type

(Sarpal et al., 2012)

Mutagen

in vitro construct

(Sarpal et al., 2012)

Progenitor genotype

Carried in construct

P{Ubi-α-Cat.S}

Cytology

Description

A ubiquitin promoter drives expression of α-Cat cDNA.

(Sarpal et al., 2012)

Allele components

Component

Use(s)

Encoded product / tool

α-Cat

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

α-Cat¹ mutant clones in the ovary (generated through recombination of the α-Cat^Ubi.PS rescue construct) display a variety of oogenesis defects. These include a compromised follicular epithelium leading to fused follicles and failure of border cell migration. The majority of α-Cat¹ mutant cells have lost contact with wild type follicle cells. These cells rarely form multilayered clusters, but are usually flattened and tightly attached to the basement membrane. α-Cat¹ mutant follicle cells appear to maintain apical-basal polarity, however neighbouring cells appear to lose polarity as their microvillus tufts point in different directions.

α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) do not develop in third instar larval imaginal discs.

(Sarpal et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU does not suppress the phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also not rescued.

Expression of α-Cat::shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Expression of α-Cat::shg^{ΔVH1.Scer\UAS.P\T} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Expression of α-Cat::shg^{ΔVH3-CTD.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also partially rescued.

Expression of α-Cat::baz^{bazOD.ΔVH1.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU does not suppress the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also not rescued.

Expression of α-Cat::baz^{ΔVH1.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are not rescued.

Expression of α-Cat::baz^{αCatNbazODC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are not rescued.

Expression of α-Cat::baz^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also partially rescued.

Expression of α-Cat::ed^{ΔVH1.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU does not suppress the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are not rescued.

(Desai et al., 2013)

Expressing shg^{Scer\UAS.P\T.cSa} does not allow α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) to develop in third instar larval imaginal discs. Mutant clone cells generated in the follicle epithelium lose cell contacts and border cells fail to migrate.

Expressing α-Cat::shg^{Scer\UAS.P\T.cSa} allows α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) to develop in third instar larval imaginal discs. The follicle epithelium and border cell migration defects are also rescued.

(Sarpal et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Ctnna2^{Scer\UAS.P\T.cDa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are partially rescued.

Expression of Mmus\Ctnna1^{Scer\UAS.P\T.cDa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are not rescued.

Expression of Mmus\Ctnna2^{Δ56.Scer\UAS.P\T} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to suppress the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are not rescued.

(Desai et al., 2013)

Complementation and Rescue Data

Partially rescued by

α-Cat^Ubi.PS is partially rescued by Scer\GAL4^Act.PU/Scer\GAL4^da.G32/α-Cat^{UASp.ΔVH2N.Tag:HA}

(Desai et al., 2013)

Comments

Expression of α-Cat^{Scer\UAS.P\T.cDa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Expression of α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH1.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). Border cell migration does not take place.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH3-CTD.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). Border cell migration does not take place.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH3.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). Border cell migration does not take place.

Expression of α-Cat^{Scer\UAS.P\T.ΔCTD.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fully rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). In most cases the border cell migration defects are also rescued.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH2.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are partially rescued.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH2N.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are partially rescued.

Expression of α-Cat^{Scer\UAS.P\T.ΔVH2C.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fully rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are almost completely rescued.

Expression of α-Cat^{Scer\UAS.P\T.ΔVIN.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU almost completely rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Expression of α-Cat^{Δ64.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU partially rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are partially rescued.

(Desai et al., 2013)

Expressing α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} allows α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) to develop in third instar larval imaginal discs. The follicle epithelium and border cell migration defects are also rescued.

(Sarpal et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (18)

Bloomington

65607

w¹¹¹⁸; P{tubP-GAL80}LL10, P{Ubi-α-Cat.S}4A P{neoFRT}40A; P{Act-GAL4.U}3, α-Cat¹/TM6B, Tb¹

65579

w¹¹¹⁸; P{tubP-GAL80}LL10, P{Ubi-α-Cat.S}4A P{neoFRT}40A; P{Act-GAL4.U}3, P{UASp-shg.S}attP2 α-Cat¹/TM6B, Tb¹

67751

w¹¹¹⁸; P{tubP-GAL80}LL10, P{Ubi-α-Cat.S}4A P{neoFRT}40A; P{Act-GAL4.U}3 P{UASp-α-Cat.ΔP}attP2 α-Cat¹/TM6B, Tb¹