FlyBase Allele Report: Dmel\α-Cat::shg[UASp.cSa]

General Information

Symbol

Dmel\α-Cat::shg^UASp.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Sarpal

FlyBase ID

FBal0294019

Feature type

allele

Associated gene

α-Cat::shg

Associated Insertion(s)

Carried in Construct

P{UASp-α-Cat::shg.fl.S}

Also Known As

DEcad::αCat

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Sarpal et al., 2012)

Progenitor genotype

α-Cat::shg^UASp.fl

(Sarpal et al., 2012)

Carried in construct

P{UASp-α-Cat::shg.fl.S}

Cytology

Description

Full-length α-Cat coding sequences are fused to the carboxy-terminal of full-length shg coding sequences. Expression is driven through UASp sequences.

(Sarpal et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

α-Cat::shg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage, with Scer\GAL4^Act.PU, Scer\GAL4^da.G32

(Sarpal et al., 2012)

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

(Desai et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of α-Cat::shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 results in early larval lethality.

(Desai et al., 2013)

Expression of α-Cat::shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU results in larval lethality.

(Sarpal et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

α-Cat::shg^UASp.cSa, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 is a suppressor of abnormal cell migration | somatic clone | adult stage phenotype of α-Cat¹

(Desai et al., 2013)

α-Cat::shg^UASp.cSa, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 is a suppressor of lethal - all die during embryonic stage phenotype of α-Cat¹

(Desai et al., 2013)

α-Cat::shg^UASp.cSa, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 is a suppressor | partially of lethal - all die during embryonic stage phenotype of α-Cat¹

(Sarpal et al., 2012)

α-Cat::shg^UASp.cSa is a suppressor of cell lethal | somatic clone | third instar larval stage phenotype of α-Cat¹

(Sarpal et al., 2012)

Other

Statement

Reference

α-Cat::shg^UASp.cSa, α-Cat¹, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 has lethal - all die before end of larval stage phenotype

(Desai et al., 2013, Sarpal et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

α-Cat::shg^UASp.cSa, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 is a suppressor of follicle cell | somatic clone phenotype of α-Cat¹

(Desai et al., 2013)

α-Cat::shg^UASp.cSa, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 is a suppressor of border follicle cell | somatic clone phenotype of α-Cat¹

(Desai et al., 2013)

α-Cat::shg^UASp.cSa, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 is a suppressor of embryonic head phenotype of α-Cat¹

(Desai et al., 2013)

α-Cat::shg^UASp.cSa, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 is a suppressor | partially of embryonic/larval cuticle | embryonic stage phenotype of α-Cat¹

(Sarpal et al., 2012)

α-Cat::shg^UASp.cSa is a suppressor of imaginal disc | somatic clone | third instar larval stage phenotype of α-Cat¹

(Sarpal et al., 2012)

α-Cat::shg^UASp.cSa is a suppressor of ovary | somatic clone phenotype of α-Cat¹

(Sarpal et al., 2012)

α-Cat::shg^UASp.cSa is a suppressor of egg chamber | somatic clone phenotype of α-Cat¹

(Sarpal et al., 2012)

α-Cat::shg^UASp.cSa is a suppressor of border follicle cell | somatic clone phenotype of α-Cat¹

(Sarpal et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of α-Cat::shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Ubiquitous expression of α-Cat::shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. Animals are unable to develop beyond the larval stages.

(Desai et al., 2013)

Expression of α-Cat::shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues head morphogenesis and embryonic lethality in 89% of α-Cat¹ mutants, but animals die during the larval stages.

Expressing α-Cat::shg^{Scer\UAS.P\T.cSa} allows α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) to develop in third instar larval imaginal discs. The follicle epithelium and border cell migration defects are also rescued.

(Sarpal et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

65580

w¹¹¹⁸; P{UASp-α-Cat::shg.fl.S}attP2

65574

w¹¹¹⁸; P{tubP-GAL80}LL10, P{Ubi-α-Cat.S}4A P{neoFRT}40A; P{Act-GAL4.U}3, P{UASp-α-Cat::shg.fl.S}attP2 α-Cat¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

α-Cat::shg^{Scer\UAS.P\T.cSa}

α-Cat::shg^UASp.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sarpal

Secondary FlyBase IDs

References (6)

Report Sections

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