FlyBase Allele Report: Dmel\α-Cat::shg[ΔVH1.UASp]

General Information

Symbol

Dmel\α-Cat::shg^ΔVH1.UASp

Species

D. melanogaster

Name

FlyBase ID

FBal0294103

Feature type

allele

Associated gene

α-Cat::shg

Associated Insertion(s)

Carried in Construct

P{UASp-α-Cat::shg.ΔVH1}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Desai et al., 2013)

Progenitor genotype

Carried in construct

P{UASp-α-Cat::shg.ΔVH1}

Cytology

Description

UASp regulatory sequences drive expression of a fusion of full-length shg and a mutant form of α-Cat lacking amino acids 1-233.

(Desai et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

α-Cat::shg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

(Desai et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of α-Cat::shg^{ΔVH1.Scer\UAS.P\T} under the control of Scer\GAL4^da.G32 results in early larval lethality.

(Desai et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, α-Cat::shg^ΔVH1.UASp is a suppressor of abnormal cell migration | somatic clone | adult stage phenotype of α-Cat¹

(Desai et al., 2013)

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, α-Cat::shg^ΔVH1.UASp is a suppressor of lethal - all die during embryonic stage phenotype of α-Cat¹

(Desai et al., 2013)

Other

Statement

Reference

α-Cat::shg^ΔVH1.UASp, α-Cat¹, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 has lethal - all die before end of larval stage phenotype

(Desai et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, α-Cat::shg^ΔVH1.UASp is a suppressor of follicle cell | somatic clone phenotype of α-Cat¹

(Desai et al., 2013)

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, α-Cat::shg^ΔVH1.UASp is a suppressor of border follicle cell | somatic clone phenotype of α-Cat¹

(Desai et al., 2013)

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, α-Cat::shg^ΔVH1.UASp is a suppressor of embryonic head phenotype of α-Cat¹

(Desai et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of α-Cat::shg^{ΔVH1.Scer\UAS.P\T} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU suppresses the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Ubiquitous expression of α-Cat::shg^{ΔVH1.Scer\UAS.P\T} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects seen in α-Cat¹ mutant embryos. Animals are unable to develop beyond the larval stages.

(Desai et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

65578

w¹¹¹⁸; P{UASp-α-Cat::shg.ΔVH1}attP2

65575

w¹¹¹⁸; P{tubP-GAL80}LL10, P{Ubi-α-Cat.S}4A P{neoFRT}40A; P{Act-GAL4.U}3, P{UASp-α-Cat::shg.ΔVH1}attP2 α-Cat¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

α-Cat::shg^{ΔVH1.Scer\UAS.P\T}

α-Cat::shg^ΔVH1.UASp

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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