FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\droshaE859K.UAS.Tag:FLAG
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General Information
Symbol
Dmel\droshaE859K.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0295072
Feature type
allele
Associated gene
Dmel\drosha
Associated Insertion(s)
Carried in Construct
P{UAS-drosha.E859K-FLAG}
Key Links
Transgenic product class
missense_variant
(Luhur et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Luhur et al., 2014)
Mutagen
in vitro construct
(Luhur et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-drosha.E859K-FLAG}
Cytology
Description

Amino acid replacement: E859K.

(Luhur et al., 2014)

UAS regulatory sequences drive expression of mutated drosha coding sequences containing a G to A change, creating a glutamic acid to lysine missense mutation in a conserved residue of the first RNase III domain. This construct is tagged at the C-terminal with Tag:FLAG.

(Luhur et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
drosha
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mushroom body clones expressing droshaE859K.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ey-OK107 appear wild-type.

      (Luhur et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4ey-OK107/droshaE859K.UAS.Tag:FLAG partially rescues drosha21K11

      (Luhur et al., 2014)
      Comments

      The rescue of drosha21K11 through expression of droshaE859K.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ey-OK107 is not complete, as α/β neuron overextension is still detected.

      (Luhur et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      droshaE859K.Scer\UAS.T:Zzzz\FLAG
      droshaE859K.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)