Insertion of a Mi{Hto-WP} element upstream of ara ( 3L:12 ,580,466 (+) -Release 6). The hostile takeover (hto) exon in the Mi{Hto-WP} element splices to the downstream ara exon 2. This results in a fusion protein being expressed under the control of Scer\UAS regulatory sequences in the Mi{Hto-WP} element. The fusion protein consists of 3 copies of the T:Zzzz\FLAG tag, the mCherry form of Disc\RFP and the ara coding sequence.
Expression of one copy of araFAR under the control of Scer\GAL4GMR.PU produces flies that have a rough eye phenotype that is more severe in the dorsal and anterior regions of the retina.
Expression of araFAR under the control of Scer\GAL4Bx-MS1096 results in fusion of wing veins L2 and L3 and a reduction in overall wing size.
Generated using the hostile takeover (Hto) system.