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General Information
Symbol
Dmel\DrgxGLO
Species
D. melanogaster
Name
FlyBase ID
FBal0295086
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion of a Mi{Hto-WP} element within Sfp24Bd ( 2L:3 ,669,091 (+) -Release 6). The hostile takeover (hto) exon in the Mi{Hto-WP} element splices to the downstream Drgx exon 2. This results in a fusion protein being expressed under the control of Scer\UAS regulatory sequences in the Mi{Hto-WP} element. The fusion protein consists of 3 copies of the T:Zzzz\FLAG tag, the mCherry form of Disc\RFP, 9 amino acid linker sequence translated from the Drgx 5'UTR exon (LGPAAAGRG) and the Drgx coding sequence. Sfp24Bd and the nearby Sfp24Ba, Sfp24Bb and Sfp24Bc genes are not induced because they are transcribed in the reverse direction.

Insertion components
Mi{Hto-WP}DrgxGLO
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of one copy of DrgxGLO under the control of Scer\GAL4GMR.PU produces flies that have severely rough eyes.

Expression of DrgxGLO under the control of Scer\GAL4ey.PU results in eye elimination and a reduced head.

Expression of DrgxGLO under the control of Scer\GAL4Bx-MS1096 suppresses wing vein development.

When DrgxGLO-expressing clones are generated in follicle cells at stages 8-10 of oogenesis under the control of Scer\GAL4Act5C.PP) the oocyte follicle cells (OFCs) fail to translocate posteriorly towards the oocyte as they do in wild type. The DrgxGLO-expressing cells over the nurse cells flatten correctly, but do not spread as they do in wild type. In posterior OFC clones, the basal circumferential actin cables become highly disorganized and the clones lack the hexagonal pattern in wild type, instead showing smooth clone borders, indicating a defect in adhesion. The apical-basal organisation of the clones is also disrupted. No cell death is observed.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Generated using the hostile takeover (Hto) system.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CG34340GLO
DrgxGLO
Name Synonyms
Secondary FlyBase IDs
    References (2)