FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Vps16Ad32
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General Information
Symbol
Dmel\Vps16Ad32
Species
D. melanogaster
Name
FlyBase ID
FBal0296357
Feature type
allele
Associated gene
Dmel\Vps16A
Associated Insertion(s)
Carried in Construct
Also Known As
Vps16A[d32]
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Takáts et al., 2014)

amorphic allele - molecular evidence

(Takáts et al., 2014)
Mutagen
P-element activity
(Takáts et al., 2014)
Progenitor genotype
P{GSV2}GS5053
(Takáts et al., 2014)
Cytology
Description

Imprecise excision of the P{GSV2} element in P{GSV2}GS5053 has resulted in a 1105bp deletion starting from the P-element insertion site, deleting the protein-coding sequences in exons 1-3 and parts of exon 4.

(Takáts et al., 2014)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:9,267,056..9,268,160 [+]
Comment:

Inferred boundaries of a 1105 bp deletion resulting from the imprecise excision of P{GSV2}GS5053, which extends from the insertion site into the Vps16A gene.

(Takáts et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Vps16Ad32 garland cells show increased numbers of autophagosomes (endogenous Atg8a-positive puncta), as compared to controls.

      (Lőrincz et al., 2019)

      Vps16Ad32/Df(3R)ED5339 mutant larvae exhibit hemocyte-derived melanotic tumors, and garland nephrocytes show fragmentation of late endosomes and many small electron dense lysosome-like organelles, as compared to controls.

      (Lőrincz et al., 2016)

      Autolysosome formation is inhibited in the fat body cells of starved Vps16Ad32/Df(3R)ED5339 mutant wandering third instar larvae.

      Adult flies containing eyes entirely composed of Vps16Ad32 mutant cells (generated using the GMR-hid method) are light orange in colour.

      (Takáts et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Vps16Ad32/Df(3R)ED5339 is rescued by Vps16A+t10.5

      (Takáts et al., 2014)
      Comments

      Expression of Vps16A+t10.5 completely restores viability and starvation-induced autolysosome defects seen in Vps16Ad32/Df(3R)ED5339 mutant wandering third instar larvae.

      (Takáts et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      References (7)