FlyBase Allele Report: Dmel\Rab14[null]

General Information

Symbol

Dmel\Rab14^null

Species

D. melanogaster

Name

FlyBase ID

FBal0296400

Feature type

allele

Associated gene

Dmel\Rab14

Associated Insertion(s)

P{?EPgy2}Rab14^null

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Garg and Wu, 2014)

Mutagen

P-element activity

(Garg and Wu, 2014)

recombination

(Garg and Wu, 2014)

Progenitor genotype

P{GT1}Rab14^BG01134

(Garg and Wu, 2014)

P{EPgy2}Rab14^EY04032

(Garg and Wu, 2014)

Associated Insertion(s)

P{?EPgy2}Rab14^null

Cytology

Description

Transposase-induced mitotic recombination between the two progenitor insertions (P{EPgy2}Rab14^EY04032 and P{GT1}Rab14^BG01134) has resulted in the deletion of the intervening sequence between them, removing the Rab14 gene. The P{GT1}Rab14^BG01134 insertion is no longer present, but a small portion of insertion sequence derived from P{EPgy2}Rab14^EY04032 remains.

(Garg and Wu, 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage

(Garg and Wu, 2014)

fertile

(Garg and Wu, 2014)

Phenotype Manifest In

hemocyte | adult stage

(Garg and Wu, 2014)

phagocytic vesicle

(Garg and Wu, 2014)

Detailed Description

Statement

Reference

Phagocytic uptake of fluorescein-conjugated S. aureus or E.coli in mutant adult hemocytes is comparable to wild type. The kinetics of maturation of phagosomes containing pHrodo-conjugated S. aureus or E.coli by mutant adult hemocytes are slower than those seen in wild type.

Mutant adults show significantly decreased survival compared to controls after injection with S. aureus, while there is no significant difference in mortality in response to wounding (injection with buffer). The mutant flies do not show susceptibility to infection with E. coli.

(Garg and Wu, 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rab14^null is rescued by Scer\GAL4^Hml.Δ/Rab14^UASp.YFP

(Garg and Wu, 2014)

Rab14^null is rescued by Rab14^{UAS.RB.mRFP(Unk)}/Scer\GAL4^Hml.Δ

(Garg and Wu, 2014)

Comments

Expression of Rab14^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Hml.Δ rescues the slower kinetics of phagosome maturation seen in Rab14^null adult hemocytes.

The increased susceptibility of Rab14^null adults to infection with S. aureus is rescued by expression of either Rab14^{Scer\UAS.P\T.T:Avic\GFP-YFP} or Rab14^{Scer\UAS.RB.T:Disc\RFP-mRFP} under the control of Scer\GAL4^Hml.Δ.

(Garg and Wu, 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rab14^null

(Mauvezin et al., 2016, Garg and Wu, 2014)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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