Homozygous CenG1A^KO.w+ mutants are viable and fertile. The mutants appear morphologically normal. Adult, larval as well pupal CenG1A^KO.w+ mutants do not show any differences in size or weight compared to wild-type controls. The relative CenG1A^KO.w+ wing area is similar to that of wild-type. Food intake is also very similar in CenG1A^KO.w+ mutants and wild-type.

Survival time of CenG1A^KO.w+ mutants under nutrient deprivation is similar to that of wild-type. Neither at the beginning nor during the starvation period CenG1A^KO.w+ mutants show obvious body fat mass differences compared to controls. No difference in fat body morphology or lipid droplet storage is observed in CenG1A^KO.w+ mutants compared to controls under standard or high-fat feeding conditions.

Whereas the timing of embryonic and first development is mostly unaffected in CenG1A^KO.w+ mutant animals, the second instar larval stage is prolonged leading to a delayed onset of pupariation. The duration of the L3 stage seems not to be affected. The onset of pupariation of CenG1A^KO.w+ mutants is delayed by one or two days relative to wild-type under both high sugar or high fat feeding conditions. A major growth delay in second instar between 45 and 80 hours after egg deposition is observed. After 80 hours, growth rate of the mutants proceeds in parallel to wild-type.

In the mutants. plateau phases in growth do not coincide with ecdysone peaks and L2/L3 or L3/pupal stage transitions.