FlyBase Allele Report: Dmel\p53[UAS.ORF.GW.Tag:HA]

General Information

Symbol

Dmel\p53^{UAS.ORF.GW.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0297129

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

M{UAS-p53.ORF.3xHA.GW}

Key Links

Transgenic product class

wild_type

(Bischof and FlyORF project members, 2014.6.20)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Bischof and FlyORF project members, 2014.6.20)

Mutagen

in vitro construct

(Bischof and FlyORF project members, 2014.6.20)

Progenitor genotype

Carried in construct

M{UAS-p53.ORF.3xHA.GW}

Cytology

Description

UAS regulatory sequences drive expression of a full-length p53 open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Bischof and FlyORF project members, 2014.6.20)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Also carries

recombinase target site

FRT5

recombinase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | male limited, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

lethal | male limited | cold sensitive, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

visible | adult stage, with Scer\GAL4^nub-AC-62

(Ikegawa et al., 2023)

visible | female limited, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

visible | female limited | cold sensitive, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

Phenotype Manifest In

wing, with Scer\GAL4^nub-AC-62

(Ikegawa et al., 2023)

wing | female limited, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

wing | female limited | cold sensitive, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

Detailed Description

Statement

Reference

Expression of p53^{Scer\UAS.ORF.GW.T:Ivir\HA1} under the control of Scer\GAL4^Bx-MS1096 results in female flies with almost complete wing loss at 18[o]C.

(Schertel et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^nub-AC-62, p53^{UAS.ORF.GW.Tag:HA} has visible | adult stage phenotype, suppressible by Scer\GAL4^nub-AC-62/synr^RNAi.UAS

(Ikegawa et al., 2023)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^nub-AC-62, p53^{UAS.ORF.GW.Tag:HA} has wing phenotype, suppressible by Scer\GAL4^nub-AC-62/synr^RNAi.UAS

(Ikegawa et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

FlyORF

F000091

M{UAS-p53.ORF.3xHA.GW}ZH-86Fb

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

p53^{Scer\UAS.ORF.GW.T:Ivir\HA1}

p53^{UAS.ORF.GW.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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