FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\wgKO.FRT.NRT-wg.FRT.wg
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General Information
Symbol
Dmel\wgKO.FRT.NRT-wg.FRT.wg
Species
D. melanogaster
Name
FlyBase ID
FBal0298269
Feature type
allele
Associated gene
Dmel\wg
Associated Insertion(s)
TI{TI}wgKO.FRT.NRT-wg.FRT.wg
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Alexandre et al., 2014)
Progenitor genotype
wgKO
(Alexandre et al., 2014)
Associated Insertion(s)
TI{TI}wgKO.FRT.NRT-wg.FRT.wg
Cytology
Description

Re-integration of DNA into the attP site present in wgKO. The reintegrated sequence is as follows: 1. an FRT cassette containing 'Nrt-wg' (a membrane-tethered, Tag:HA-tagged form of wg in which Tag:M(Nrt) acts as the membrane tethering sequence), 2. wild-type wg cDNA sequence, and 3. a loxP cassette containing a Disc\RFPmCherry.3xP3 marker. The wild-type wg sequence is not expressed due to the presence of the upstream FRT cassette. In addition, a recombined attP/B site is present 5' to the reintegrated sequence.

(Alexandre et al., 2014)
Allele components
Component
Use(s)
Tagged with
Tag:HA
Tag:M(Nrt)
Also carries
FRT
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      "wg{KO; NRT-Wg[Body]; WT[Discs]}" larvae expressing wild-type wg in imaginal discs and 'NRT-wg' (a membrane-tethered form of wg) in other tissues have been generated by expressing FLPase in the imaginal discs of homozygous wgKO;FRT.NRT-wg.FRT.wg larvae (converting wgKO;FRT.NRT-wg.FRT.wg to wgKO;-FRT.wg in the discs). These animals largely fail to eclose when co-cultured with control siblings, but eclose into normal-looking adults when grown in separate vials.

      (Alexandre et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      FRT NRT-Wg FRT Wg
      (Alexandre et al., 2014)
      wgKO.FRT.NRT-wg.FRT.wg
      wgKO;FRT.NRT-wg.FRT.wg
      Name Synonyms
      Secondary FlyBase IDs
        References (4)