FB2026_02 , released June 18, 2026
Allele: Dmel\Usp5ED.UAS.Tag:FLAG
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General Information
Symbol
Dmel\Usp5ED.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0298998
Feature type
allele
Associated gene
Dmel\Usp5
Associated Insertion(s)
Carried in Construct
P{UAS-3xFlag.Usp5.ED}
Also Known As
UAS-Flag-ED-leon, UAS-ED-leon
Key Links
Transgenic product class
missense_variant
(Wang et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Wang et al., 2014)
Mutagen
in vitro construct
(Wang et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-3xFlag.Usp5.ED}
Cytology
Description

UAS regulates expression of the Usp5 cDNA in which Cys341 is substituted with Ala in the catalytic site to render it 'enzyme dead (ED)', with 3 Tag:FLAG repeats fused to the N-terminus.

(Wang et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Usp5
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4how-24B/Usp5ED.UAS.Tag:FLAG fails to rescue Usp519-2/Usp5leon-1

      (Wang et al., 2017)

      Scer\GAL4Toll-6-D42/Usp5ED.UAS.Tag:FLAG fails to rescue Usp519-2/Usp5leon-1

      (Wang et al., 2017)

      Scer\GAL4Tub.PU/Usp5ED.UAS.Tag:FLAG fails to rescue Usp519-2/Usp5leon-1

      (Wang et al., 2014)
      Comments

      The neuromuscular junction morphological defects and reduced number of boutons observed in Usp5leon-1/Usp519-2 mutant third instar larvae cannot be rescued by expression of Usp5ED.Scer\UAS.T:Zzzz\FLAG be it driven by either Scer\GAL4how-24B or Scer\GAL4Toll-6-D42.

      (Wang et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Usp5ED.Scer\UAS.T:Zzzz\FLAG
      Usp5ED.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (4)