FB2026_03 , released September 17, 2026
Recombinant Construct: P{UAS-3xFlag.Usp5.ED}
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General Information
Symbol
P{UAS-3xFlag.Usp5.ED}
FlyBase ID
FBtp0095520
Feature type
transgenic_transposable_element
Component Allele(s)
Usp5ED.UAS.Tag:FLAG
Also Known As
UAS-Flag-ED-leon, UAS-ED-leon
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Usp5ED.UAS.Tag:FLAG
Transgenic product class
missense_variant
(Wang et al., 2014)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Usp5
Tagged with
Tag:FLAG
Description

UAS regulates expression of the Usp5 cDNA in which Cys341 is substituted with Ala in the catalytic site to render it 'enzyme dead (ED)', with 3 Tag:FLAG repeats fused to the N-terminus.

(Wang et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
P{UAS-3xFlag.Usp5.ED}
(FlyBase, 1992-)
UAS-3xFlag-ED-leon
(Wang et al., 2014)
UAS-ED-leon
(Wang et al., 2017, Wang et al., 2014)
UAS-Flag-ED-Leon
(Pai et al., 2023)
UAS-Flag-ED-leon
(Komori et al., 2025, Wang et al., 2017)
flag::usp5C341A
(Komori et al., 2025)
Secondary FlyBase IDs
    References (6)